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Literature summary for 4.1.1.31 extracted from
- Purification and characterization of phosphoenolpyruvate carboxylase from a cyanobacterium (1986), J. Bacteriol., 168, 207-212.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 3-Phosphoglyceric acid | - |
Coccochloris peniocystis |  |
| pyruvate | activates | Coccochloris peniocystis | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-oxoglutarate | - |
Coccochloris peniocystis | |
| alpha-Hydroxy-2-pyridylmethanesulfonate | - |
Coccochloris peniocystis | |
| ATP | - |
Coccochloris peniocystis | |
| citrate | - |
Coccochloris peniocystis | |
| isocitrate | - |
Coccochloris peniocystis | |
| Maleate | - |
Coccochloris peniocystis | |
| malonate | - |
Coccochloris peniocystis | |
| oxaloacetate | - |
Coccochloris peniocystis | |
| phosphate | - |
Coccochloris peniocystis | |
| Picolinic acid | - |
Coccochloris peniocystis | |
| quinolinic acid | - |
Coccochloris peniocystis | |
| SO42- | - |
Coccochloris peniocystis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.6 | - |
phosphoenolpyruvate | - |
Coccochloris peniocystis | |
| 0.8 | - |
HCO3- | - |
Coccochloris peniocystis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 60% of the activation with Mg2+ | Coccochloris peniocystis | |
| Mg2+ | activates | Coccochloris peniocystis | |
| Mg2+ | Km: 0.27 mM | Coccochloris peniocystis | |
| Mn2+ | 80% of the activation with Mg2+ | Coccochloris peniocystis | |
| NaCl | activation between 25 and 200 mM to a maximum of 14% a at 75 mM | Coccochloris peniocystis | |
| Zn2+ | 60% of the activation with Mg2+ | Coccochloris peniocystis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 560000 | - |
gel filtration | Coccochloris peniocystis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Coccochloris peniocystis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Coccochloris peniocystis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8.87 | - |
- |
Coccochloris peniocystis |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°C, concentrated, undesalted solution containing 33% v/v glycerol, stable for at least 6 weeks | Coccochloris peniocystis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| phosphoenolpyruvate + CO2 | - |
Coccochloris peniocystis | phosphate + oxaloacetate | - |
? | |
| phosphoenolpyruvate + HCO3- | - |
Coccochloris peniocystis | phosphate + oxaloacetate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Coccochloris peniocystis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Coccochloris peniocystis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | activates | Coccochloris peniocystis | |
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