Activating Compound | Comment | Organism | Structure |
---|---|---|---|
dioxane | stimulates | Thiobacillus thioparus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Asp | L-Asp, competitive | Thiobacillus thioparus | |
malate | competitive; L-malate | Thiobacillus thioparus | |
phosphate | orthophosphate is noncompetitive with phosphoenolpyruvate | Thiobacillus thioparus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.44 | - |
phosphoenolpyruvate | - |
Thiobacillus thioparus | |
0.89 | - |
HCO3- | - |
Thiobacillus thioparus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | Km: 0.37 mM | Thiobacillus thioparus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thiobacillus thioparus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thiobacillus thioparus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.32 | - |
- |
Thiobacillus thioparus |
Storage Stability | Organism |
---|---|
-18°C, 10 mM 2-mercaptoethanol, stable for several weeks | Thiobacillus thioparus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphoenolpyruvate + CO2 | - |
Thiobacillus thioparus | phosphate + oxaloacetate | - |
? | |
phosphoenolpyruvate + HCO3- | - |
Thiobacillus thioparus | phosphate + oxaloacetate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.3 | - |
tricine buffer | Thiobacillus thioparus |
7.4 | - |
Tris-HCl buffer | Thiobacillus thioparus |