KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.4 | - |
L-tryptophan | at pH 9.0 and 30°C | Micrococcus percitreus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
101000 | - |
gel filtration | Micrococcus percitreus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tryptophan | Micrococcus percitreus | 100% activity | tryptamine + CO2 | - |
? | |
L-tryptophan | Micrococcus percitreus AJ 1065 | 100% activity | tryptamine + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Micrococcus percitreus | - |
- |
- |
Micrococcus percitreus AJ 1065 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE-Sephadex column chromatography, and Sephadex G-200 gel filtration | Micrococcus percitreus |
Storage Stability | Organism |
---|---|
5°C, 0.5 M potassium phosphate buffer pH 7.0 containing 20% (v/v) glycerol and 10 mM pyridoxal phosphate, more than 10 months, no loss of activity | Micrococcus percitreus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-chloro-L-tyrosine | 77% activity compared to L-tryptophan | Micrococcus percitreus | ? | - |
? | |
2-methyl-L-tyrosine | 75% activity compared to L-tryptophan | Micrococcus percitreus | ? | - |
? | |
3,4-Dihydroxy-L-phenylalanine | 10% activity compared to L-tryptophan | Micrococcus percitreus | ? | - |
? | |
3,4-Dihydroxy-L-phenylalanine | 10% activity compared to L-tryptophan | Micrococcus percitreus AJ 1065 | ? | - |
? | |
3,5-dibromo-DL-tyrosine | 72% activity compared to L-tryptophan | Micrococcus percitreus | ? | - |
? | |
3-chloro-L-tyrosine | 81% activity compared to L-tryptophan | Micrococcus percitreus | ? | - |
? | |
3-Hydroxy-DL-kynurenine | 8% activity compared to L-tryptophan | Micrococcus percitreus | ? | - |
? | |
3-Hydroxy-DL-kynurenine | 8% activity compared to L-tryptophan | Micrococcus percitreus AJ 1065 | ? | - |
? | |
3-methyl-L-tyrosine | 80% activity compared to L-tryptophan | Micrococcus percitreus | ? | - |
? | |
5-fluoro-DL-tryptophan | 75% activity compared to L-tryptophan | Micrococcus percitreus | ? | - |
? | |
5-hydroxy-L-tryptophan | 24% activity compared to L-tryptophan | Micrococcus percitreus | ? | - |
? | |
5-methyl-DL-tryptophan | 17% activity compared to L-tryptophan | Micrococcus percitreus | ? | - |
? | |
7-aza-DL-tryptophan | 8% activity compared to L-tryptophan | Micrococcus percitreus | ? | - |
? | |
alpha-methyl-DL-phenylalanine | 2% activity compared to L-tryptophan | Micrococcus percitreus | ? | - |
? | |
alpha-methyl-DL-tyrosine | 1% activity compared to L-tryptophan | Micrococcus percitreus | ? | - |
? | |
L-2,4-dihydroxyphenylalanine | 75% activity compared to L-tryptophan | Micrococcus percitreus | ? | - |
? | |
L-kynurenine | 15% activity compared to L-tryptophan | Micrococcus percitreus | ? | - |
? | |
L-phenylalanine | 120% activity compared to L-tryptophan | Micrococcus percitreus | ? | - |
? | |
L-tryptophan | 100% activity | Micrococcus percitreus | tryptamine + CO2 | - |
? | |
L-tryptophan | 100% activity | Micrococcus percitreus AJ 1065 | tryptamine + CO2 | - |
? | |
L-tyrosine | 87% activity compared to L-tryptophan | Micrococcus percitreus | ? | - |
? | |
L-tyrosine | 87% activity compared to L-tryptophan | Micrococcus percitreus AJ 1065 | ? | - |
? | |
m-DL-tyrosine | 5% activity compared to L-tryptophan | Micrococcus percitreus | ? | - |
? | |
additional information | no activity with 4-nitro-DL-phenylalanine, 3,5-dinitro-DL-tyrosine, 2,4,4-trihydroxyphenyl-L-alanine, alpha-methyl-3,4-dihydroxy-phenyl-L-alanine, 2-phenyl-DL-serine, 2-thiazole-DL-alanine, and L-histidine | Micrococcus percitreus | ? | - |
? | |
additional information | no activity with 4-nitro-DL-phenylalanine, 3,5-dinitro-DL-tyrosine, 2,4,4-trihydroxyphenyl-L-alanine, alpha-methyl-3,4-dihydroxy-phenyl-L-alanine, 2-phenyl-DL-serine, 2-thiazole-DL-alanine, and L-histidine | Micrococcus percitreus AJ 1065 | ? | - |
? | |
o-DL-tyrosine | 32% activity compared to L-tryptophan | Micrococcus percitreus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 48000, SDS-PAGE | Micrococcus percitreus |
Synonyms | Comment | Organism |
---|---|---|
Aromatic L-amino acid decarboxylase | - |
Micrococcus percitreus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | 50 | the purified enzyme is stable until 35°C for 60 min but rapidly inactivated at above 50°C | Micrococcus percitreus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Micrococcus percitreus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | the enzyme is stable for 60 min between pH 6.0 and 7.0 | Micrococcus percitreus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Micrococcus percitreus |