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Literature summary for 4.1.1.23 extracted from
- Structure and inhibition of orotidine 5-monophosphate decarboxylase from Plasmodium falciparum (2008), Biochemistry, 47, 3842-3854.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into the vector pET3d for expression in Escherichia coli cells | Plasmodium falciparum |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| the structure of PfODCase is solved to a resolution of 1.5418 A | Plasmodium falciparum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 6-azauridine 5'-monophosphate | - |
Plasmodium falciparum |  |
| allopurinol-3-riboside 5'-monophosphate | - |
Plasmodium falciparum | |
| pyrazofurin 5'-monophosphate | - |
Plasmodium falciparum | |
| xanthosine 5'-monophosphate | - |
Plasmodium falciparum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00035 | - |
orotidine 5'-phosphate | - |
Plasmodium falciparum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
subunit, determined by SDS-PAGE | Plasmodium falciparum |
| 76000 | - |
determined by gel filtration | Plasmodium falciparum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Orotidine 5'-phosphate | Plasmodium falciparum | - |
UMP + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Plasmodium falciparum | Q8T6J6 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using a Ni-NTA agarose column, the polyhistidine tag is removed with thrombin, the cleaved protein is further purified using a POROS-HS cation exchange and a Superdex-200 size-exclusion column | Plasmodium falciparum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Orotidine 5'-phosphate | - |
Plasmodium falciparum | UMP + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | - |
Plasmodium falciparum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Orotidine 5'-monophosphate decarboxylase | - |
Plasmodium falciparum |
| PfODCase | - |
Plasmodium falciparum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
activity assay | Plasmodium falciparum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
activity assay | Plasmodium falciparum |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0000036 | - |
pyrazofurin 5'-monophosphate | - |
Plasmodium falciparum | |
| 0.0000044 | - |
xanthosine 5'-monophosphate | - |
Plasmodium falciparum | |
| 0.000012 | - |
6-azauridine 5'-monophosphate | - |
Plasmodium falciparum | |
| 0.00024 | - |
allopurinol-3-riboside 5'-monophosphate | - |
Plasmodium falciparum | |
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