Literature summary for 4.1.1.23 extracted from

Krungkrai, S.R.; Tokuoka, K.; Kusakari, Y.; Inoue, T.; Adachi, H.; Matsumura, H.; Takano, K.; Murakami, S.; Mori, Y.; Kai, Y.; Krungkrai, J.; Horii, T.
Crystallization and preliminary crystallographic analysis of orotidine 5-monophosphate decarboxylase from the human malaria parasite Plasmodium falciparum (2006), Acta Crystallogr. Sect. F, F62, 542-545.

Search for more literature for 4.1.1.23

Cloned(Commentary)

Cloned (Comment)	Organism
functional enzyme expression in Escherichia coli	Plasmodium falciparum

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant enzyme, seeding method in a hanging drop, 20°C, 10 mg/ml protein in in 50 mM Tris-HCl, pH 8.0, containing 300 mM NaCl and 5 mM dithiothreitol, method optimization, X-ray diffraction structure determination and analysis at 2.7 A resolution	Plasmodium falciparum

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
38000	-	2 * 38000, active recombinant enzyme, SDS-PAGE	Plasmodium falciparum
76000	-	active recombinant enzyme, gel filtration	Plasmodium falciparum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
Orotidine 5'-phosphate	Plasmodium falciparum	final step in the de novo synthesis of uridine 5'-monophosphate, UMP, defects in the enzyme are lethal in the malaria parasite	UMP + CO2	-	?

Organism

Organism	UniProt	Comment	Textmining
Plasmodium falciparum	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
functional recombinant enzyme 300fold from Escherichia coli by nickel affinity chromatography and gel filtration	Plasmodium falciparum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
10	-	purified recombinant enzyme	Plasmodium falciparum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Orotidine 5'-phosphate	-	Plasmodium falciparum	UMP + CO2	-	?
Orotidine 5'-phosphate	final step in the de novo synthesis of uridine 5'-monophosphate, UMP, defects in the enzyme are lethal in the malaria parasite	Plasmodium falciparum	UMP + CO2	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 38000, active recombinant enzyme, SDS-PAGE	Plasmodium falciparum

Synonyms

Synonyms	Comment	Organism
OMP decarboxylase	-	Plasmodium falciparum
OMPDC	-	Plasmodium falciparum
orotidine 5-monophosphate decarboxylase	-	Plasmodium falciparum

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Release 2023.1 (January 2023)