Cloned (Comment) | Organism |
---|---|
genes gadA and gadB, co-overexpression of the enzyme fused to a protein-protein interaction domain SH3 in Escherichia coli strain XL1-Blue with gene gadC encoding an antiporter | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a synthetic protein complex to improve the 4-aminobutanoate conversion in engineered Escherichia coli strain XL1-Blue, by assembling a single protein-protein interaction domain SH3 to the glutamate decarboxylase (GadA and GadB) and attaching a cognate peptide ligand to the glutamate/4-aminobutanoate antiporter (GadC) at the N-terminus, C-terminus, and the 233rd amino acid residue | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate | Escherichia coli | - |
4-aminobutanoate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
genes gadA and gadB | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
4-aminobutanoate concentrations of 4.34-5.65 g/l in engineered Escherichia coli expressing enzymes GadA or GadB with antiporter GadC from different constructs, pH 6.2, 37°C | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate | - |
Escherichia coli | 4-aminobutanoate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GadA | - |
Escherichia coli |
GadB | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.2 | - |
assay at | Escherichia coli |