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Literature summary for 4.1.1.15 extracted from

  • Le Vo, T.D.; Ko, J.S.; Park, S.J.; Lee, S.H.; Hong, S.H.
    Efficient gamma-aminobutyric acid bioconversion by employing synthetic complex between glutamate decarboxylase and glutamate/GABA antiporter in engineered Escherichia coli (2013), J. Ind. Microbiol. Biotechnol., 40, 927-933.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
genes gadA and gadB, co-overexpression of the enzyme fused to a protein-protein interaction domain SH3 in Escherichia coli strain XL1-Blue with gene gadC encoding an antiporter Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information construction of a synthetic protein complex to improve the 4-aminobutanoate conversion in engineered Escherichia coli strain XL1-Blue, by assembling a single protein-protein interaction domain SH3 to the glutamate decarboxylase (GadA and GadB) and attaching a cognate peptide ligand to the glutamate/4-aminobutanoate antiporter (GadC) at the N-terminus, C-terminus, and the 233rd amino acid residue Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate Escherichia coli
-
4-aminobutanoate + CO2
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
genes gadA and gadB
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
4-aminobutanoate concentrations of 4.34-5.65 g/l in engineered Escherichia coli expressing enzymes GadA or GadB with antiporter GadC from different constructs, pH 6.2, 37°C Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate
-
Escherichia coli 4-aminobutanoate + CO2
-
?

Synonyms

Synonyms Comment Organism
GadA
-
Escherichia coli
GadB
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2
-
assay at Escherichia coli