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Literature summary for 4.1.1.15 extracted from

  • Park, H.; Ahn, J.; Lee, J.; Lee, H.; Kim, C.; Jung, J.K.; Lee, H.; Lee, E.G.
    Expression, immobilization and enzymatic properties of glutamate decarboxylase fused to a cellulose-binding domain (2012), Int. J. Mol. Sci., 13, 358-368.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.1.15

Application

Application Comment Organism
synthesis the enzyme is a key component of 4-aminobutanoate production through an enzymatic process Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
gene gad, the enzyme is fused to the cellulose-binding domain and a linker of Trichoderma harzianum endoglucanase II, or S3N10 peptide replacing the native linker to prevent proteolytic cleavage, functional expression of the His6-tagged fusion construct in Escherichia coli strain BL21(DE3) Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information immobilization of the cellulose binding domain-enzyme fusion protein on a crystalline cellulose resin, with binding capacity of 33 nmol CBD-GAD/g resin, the immobilized enzymes retains 60% of initial activiy after 10 uses Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
 x * 58000, recombinant His6-tagged fusion enzyme, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate Escherichia coli
-
 4-aminobutanoate + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged fusion enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate
-
 Escherichia coli 4-aminobutanoate + CO2
-
 ?

Subunits

Subunits Comment Organism
? x * 58000, recombinant His6-tagged fusion enzyme, SDS-PAGE Escherichia coli

Synonyms

Synonyms Comment Organism
GAD
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4
-
 assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Escherichia coli