Cloned (Comment) | Organism |
---|---|
gene gad, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Levilactobacillus brevis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AgNO3 | - |
Levilactobacillus brevis | |
CuCl2 | - |
Levilactobacillus brevis | |
KI | - |
Levilactobacillus brevis | |
Na2SO4 | - |
Levilactobacillus brevis | |
ZnCl2 | - |
Levilactobacillus brevis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.6 | - |
L-glutamate | pH 5.2, 45°C, recombinant enzyme | Levilactobacillus brevis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | potent activation | Levilactobacillus brevis | |
additional information | no effect on activity by NaCl, MgCl2, and MnCl2 | Levilactobacillus brevis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
x * 53522, sequence calculation, x * 50000, recombinant His-tagged enzyme, SDS-PAGE | Levilactobacillus brevis |
53522 | - |
x * 53522, sequence calculation, x * 50000, recombinant His-tagged enzyme, SDS-PAGE | Levilactobacillus brevis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate | Levilactobacillus brevis | - |
4-aminobutanoate + CO2 | - |
? | |
L-glutamate | Levilactobacillus brevis 877G | - |
4-aminobutanoate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Levilactobacillus brevis | K4HXK6 | isolated from traditional Korean fermented food kimchi, gene gad | - |
Levilactobacillus brevis 877G | K4HXK6 | isolated from traditional Korean fermented food kimchi, gene gad | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Levilactobacillus brevis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | strain 877G, isolated from traditional Korean fermented food kimchi, shows very high gamma-aminobutanoate production | Levilactobacillus brevis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate | - |
Levilactobacillus brevis | 4-aminobutanoate + CO2 | - |
? | |
L-glutamate | - |
Levilactobacillus brevis 877G | 4-aminobutanoate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 53522, sequence calculation, x * 50000, recombinant His-tagged enzyme, SDS-PAGE | Levilactobacillus brevis |
Synonyms | Comment | Organism |
---|---|---|
LbGAD | - |
Levilactobacillus brevis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
recombinant enzyme | Levilactobacillus brevis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.286 | - |
L-glutamate | pH 5.2, 45°C, recombinant enzyme | Levilactobacillus brevis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.2 | - |
recombinant enzyme | Levilactobacillus brevis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | residue K279 is essential for cofactor binding | Levilactobacillus brevis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Levilactobacillus brevis | sequence calculation | - |
5.4 |
General Information | Comment | Organism |
---|---|---|
additional information | residues T215 and D246 are involved in catalysis | Levilactobacillus brevis |