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Literature summary for 4.1.1.15 extracted from

  • Seo, M.J.; Nam, Y.D.; Lee, S.Y.; Park, S.L.; Yi, S.H.; Lim, S.I.
    Expression and characterization of a glutamate decarboxylase from Lactobacillus brevis 877G producing gamma-aminobutyric acid (2013), Biosci. Biotechnol. Biochem., 77, 853-856.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene gad, DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Levilactobacillus brevis

Inhibitors

Inhibitors Comment Organism Structure
AgNO3
-
Levilactobacillus brevis
CuCl2
-
Levilactobacillus brevis
KI
-
Levilactobacillus brevis
Na2SO4
-
Levilactobacillus brevis
ZnCl2
-
Levilactobacillus brevis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6
-
L-glutamate pH 5.2, 45°C, recombinant enzyme Levilactobacillus brevis

Metals/Ions

Metals/Ions Comment Organism Structure
CaCl2 potent activation Levilactobacillus brevis
additional information no effect on activity by NaCl, MgCl2, and MnCl2 Levilactobacillus brevis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
x * 53522, sequence calculation, x * 50000, recombinant His-tagged enzyme, SDS-PAGE Levilactobacillus brevis
53522
-
x * 53522, sequence calculation, x * 50000, recombinant His-tagged enzyme, SDS-PAGE Levilactobacillus brevis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-glutamate Levilactobacillus brevis
-
4-aminobutanoate + CO2
-
?
L-glutamate Levilactobacillus brevis 877G
-
4-aminobutanoate + CO2
-
?

Organism

Organism UniProt Comment Textmining
Levilactobacillus brevis K4HXK6 isolated from traditional Korean fermented food kimchi, gene gad
-
Levilactobacillus brevis 877G K4HXK6 isolated from traditional Korean fermented food kimchi, gene gad
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Levilactobacillus brevis

Source Tissue

Source Tissue Comment Organism Textmining
additional information strain 877G, isolated from traditional Korean fermented food kimchi, shows very high gamma-aminobutanoate production Levilactobacillus brevis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate
-
Levilactobacillus brevis 4-aminobutanoate + CO2
-
?
L-glutamate
-
Levilactobacillus brevis 877G 4-aminobutanoate + CO2
-
?

Subunits

Subunits Comment Organism
? x * 53522, sequence calculation, x * 50000, recombinant His-tagged enzyme, SDS-PAGE Levilactobacillus brevis

Synonyms

Synonyms Comment Organism
LbGAD
-
Levilactobacillus brevis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
recombinant enzyme Levilactobacillus brevis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.286
-
L-glutamate pH 5.2, 45°C, recombinant enzyme Levilactobacillus brevis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2
-
recombinant enzyme Levilactobacillus brevis

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate residue K279 is essential for cofactor binding Levilactobacillus brevis

pI Value

Organism Comment pI Value Maximum pI Value
Levilactobacillus brevis sequence calculation
-
5.4

General Information

General Information Comment Organism
additional information residues T215 and D246 are involved in catalysis Levilactobacillus brevis