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Literature summary for 4.1.1.15 extracted from

  • Molina-Rueda, J.J.; Pascual, M.B.; Canovas, F.M.; Gallardo, F.
    Characterization and developmental expression of a glutamate decarboxylase from maritime pine (2010), Planta, 232, 1471-1483.
    View publication on PubMed
Search for more literature for 4.1.1.15

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-codonPlus-(DE3)-RIL cells Pinus pinaster

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ the addition of Ca2+ to the reaction mix causes an activation of GAD activity of 32% Pinus pinaster

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 x * 56000, SDS-PAGE Pinus pinaster
57200
-
 x * 57200, calculated from amino acid sequence Pinus pinaster

Organism

Organism UniProt Comment Textmining
Pinus pinaster A0EJ89
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Pinus pinaster

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
 Pinus pinaster
-
embryo
-
 Pinus pinaster
-
hypocotyl
-
 Pinus pinaster
-
root highest expression Pinus pinaster
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate
-
 Pinus pinaster 4-aminobutanoate + CO2
-
 ?

Subunits

Subunits Comment Organism
? x * 56000, SDS-PAGE Pinus pinaster
? x * 57200, calculated from amino acid sequence Pinus pinaster

Synonyms

Synonyms Comment Organism
GAD
-
 Pinus pinaster

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
-
-
 Pinus pinaster

Cofactor

Cofactor Comment Organism Structure
Calmodulin GAD can be regulated in vivo by Ca2+/calmodulin atpH 5.8. The addition of bovine calmodulin and Ca2+ to the reaction mix causes an activation of GAD activity of 32% Pinus pinaster
pyridoxal 5'-phosphate
-
 Pinus pinaster

pI Value

Organism Comment pI Value Maximum pI Value
Pinus pinaster calculated from amino acid sequence
-
 5.7

Expression

Organism Comment Expression
Pinus pinaster GAD expression is induced in response to the production of compression wood and GAD increases during day 14 and 82 after germination in the hypocotyl up

General Information

General Information Comment Organism
physiological function GAD plays a role in hypocotyl and stem development in pine Pinus pinaster