Literature summary for 4.1.1.15 extracted from

Yao, W.; Wu, X.; Zhu, J.; Sun, B.; Miller, C.
System establishment of ATPS for one-step purification of glutamate decarboxylase from E. coli after cell disruption (2011), Appl. Biochem. Biotechnol., 164, 1339-1349.

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Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
in polyethylene glycol (PEG) and sodium sulfate aqueous two-phase system. The optimum system obtained for GAD purification is composed of PEG 4000, tie line length of 63.5%, a volume ratio of 2.31, a loading sample concentration of 0.4 g/ml, which produces a GAD recovery of 90% with the purification fold of 73. The purification fold declines from 71.73 to 23.27 with the addition of 1 mM NaCl	Escherichia coli

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-glutamate	-	Escherichia coli	4-aminobutanoate + CO2	-	?

Synonyms

Synonyms	Comment	Organism
GAD	-	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
pyridoxal 5'-phosphate	-	Escherichia coli

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Release 2023.1 (January 2023)