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Literature summary for 4.1.1.15 extracted from

  • Gut, H.; Dominici, P.; Pilati, S.; Astegno, A.; Petoukhov, M.V.; Svergun, D.I.; Gruetter, M.G.; Capitani, G.
    A common structural basis for pH- and calmodulin-mediated regulation in plant glutamate decarboxylase (2009), J. Mol. Biol., 392, 334-351.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
Calmodulin activates Gad1 in a unique way by relieving two C-terminal autoinhibition domains of adjacent active sites, forming a 393 kDa Gad-Ca2+/calmodulin complex with a 1:3 stoichiometry Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Arabidopsis thaliana

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 100 mM sodium acetate pH 5.5, 720 mM sodium formate, 9% (w/v) PEG 8000, and 9% (w/v) PEG 1000 Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
K496A/K497A the mutant displays a substantial loss of enzymatic activity, at the pH optimum, the activity of the double mutant is reduced about 3fold Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57066
-
X-ray crystallography, 6 * 57066, amino acid sequence Arabidopsis thaliana
310000
-
static light-scattering Arabidopsis thaliana
342000
-
calculated from amino acid sequence Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q42521
-
-

Purification (Commentary)

Purification (Comment) Organism
CaM agarose column chromatography and Superdex 200 gel filtration Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate
-
Arabidopsis thaliana 4-aminobutanoate + CO2
-
?

Subunits

Subunits Comment Organism
homohexamer X-ray crystallography, 6 * 57066, amino acid sequence Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
GAD1
-
Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
maximal turnover in the absence of calmodulin Arabidopsis thaliana
6
-
maximum activity at about pH 6 Arabidopsis thaliana

pH Stability

pH Stability pH Stability Maximum Comment Organism
7
-
Gad1 is not completely inactive at physiological pH and its residual activity is essential for normal plant development Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Arabidopsis thaliana