Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Calmodulin | activates Gad1 in a unique way by relieving two C-terminal autoinhibition domains of adjacent active sites, forming a 393 kDa Gad-Ca2+/calmodulin complex with a 1:3 stoichiometry | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Arabidopsis thaliana |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 100 mM sodium acetate pH 5.5, 720 mM sodium formate, 9% (w/v) PEG 8000, and 9% (w/v) PEG 1000 | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
K496A/K497A | the mutant displays a substantial loss of enzymatic activity, at the pH optimum, the activity of the double mutant is reduced about 3fold | Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57066 | - |
X-ray crystallography, 6 * 57066, amino acid sequence | Arabidopsis thaliana |
310000 | - |
static light-scattering | Arabidopsis thaliana |
342000 | - |
calculated from amino acid sequence | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q42521 | - |
- |
Purification (Comment) | Organism |
---|---|
CaM agarose column chromatography and Superdex 200 gel filtration | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamate | - |
Arabidopsis thaliana | 4-aminobutanoate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homohexamer | X-ray crystallography, 6 * 57066, amino acid sequence | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
GAD1 | - |
Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
maximal turnover in the absence of calmodulin | Arabidopsis thaliana |
6 | - |
maximum activity at about pH 6 | Arabidopsis thaliana |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
Gad1 is not completely inactive at physiological pH and its residual activity is essential for normal plant development | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Arabidopsis thaliana |