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Literature summary for 4.1.1.15 extracted from
- Characterization of a novel glutamate decarboxylase from Streptococcus salivarius ssp. thermophilus Y2 (2008), J. Chem. Technol. Biotechnol., 83, 855-861.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene gadB, DNA and amino acid sequence determination and analysis | Streptococcus thermophilus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AgNO3 | complete inhibition at 5mM | Streptococcus thermophilus |  |
| CoCl2 | 62% inhibition at 5mM | Streptococcus thermophilus | |
| CuSO4 | 93% inhibition at 5mM | Streptococcus thermophilus | |
| FeSO4 | 92% inhibition at 5mM | Streptococcus thermophilus | |
| MnSO4 | 88% inhibition at 5mM | Streptococcus thermophilus | |
| additional information | poor inhibition by LiCl, CaCl2, and KCl. MgCl2 and NaCl have no effect on enzyme activity | Streptococcus thermophilus | |
| Na2SO4 | 10% inhibition at 5mM | Streptococcus thermophilus | |
| ZnSO4 | 80.4% inhibition at 5mM | Streptococcus thermophilus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.3 | - |
L-glutamate | pH 4.0, 40°C | Streptococcus thermophilus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| BaCl2 | activates by 22% at 5 mM | Streptococcus thermophilus | |
| additional information | MgCl2 and NaCl have no effect on enzyme activity | Streptococcus thermophilus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46900 | - |
2 * 46900, SDS-PAGE | Streptococcus thermophilus |
| 103600 | - |
gel filtration | Streptococcus thermophilus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate | Streptococcus thermophilus | - |
4-aminobutanoate + CO2 | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptococcus thermophilus | Q0GE18 | ssp. thermophilus Y2, gene gadB | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 22fold to homogeneity by ammonium sulfate fractionation, precipitation in N-methylpiperazine, anion exchange and hydrophobic interaction chromatography, and gel filtration | Streptococcus thermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 103.5 | - |
purified enzyme | Streptococcus thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-glutamate | - |
Streptococcus thermophilus | 4-aminobutanoate + CO2 | - |
ir | |
| L-glutamate | irreversible alpha-decarboxylation of L-glutamate, the enzyme is highly specific for L-glutamate, no activity with D-glutamate and 23 other amino acids, overview | Streptococcus thermophilus | 4-aminobutanoate + CO2 | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 46900, SDS-PAGE | Streptococcus thermophilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GAD | - |
Streptococcus thermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Streptococcus thermophilus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 80 | about 40% of maximal activity at 40°C and 80°C | Streptococcus thermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
purified enzyme, pH 4.0, loss of 10% activity after 5 h, stable below | Streptococcus thermophilus |
| 50 | - |
purified enzyme, pH 4.0, loss of 3% activity after 1 h | Streptococcus thermophilus |
| 60 | - |
purified enzyme, pH 4.0, loss of about 15% activity after 5 h | Streptococcus thermophilus |
| 80 | - |
purified enzyme, pH 4.0, loss of about 50% activity after 5 h | Streptococcus thermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
- |
Streptococcus thermophilus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 5.2 | pH 3.5: about 40% of maximal activity, pH 4.5: about 65% of maximal activity, pH 5.0: about 50% of maximal activity pH 5.2: about 40% of maximal activity | Streptococcus thermophilus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | - |
purified enzyme, loss of about 35% activity at 40°C after 5 h | Streptococcus thermophilus |
| 4 | - |
purified enzyme, 40°C, loss of 3% activity after 1 h, and of 10% activity after 5 h | Streptococcus thermophilus |
| 4 | - |
purified enzyme, maximally stable at pH 4.0 | Streptococcus thermophilus |
| 5.5 | - |
purified enzyme, loss of about 40% activity at 40°C after 5 h | Streptococcus thermophilus |
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