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Literature summary for 4.1.1.15 extracted from

  • Sha, D.; Wei, J.; Wu, H.; Jin, Y.; Wu, J.Y.
    Molecular cloning, expression, purification, and characterization of shorter forms of human glutamic decarboxylase 67 in an E. coli expression system (2005), Brain Res. Mol. Brain Res., 136, 255-261.
    View publication on PubMed

Protein Variants

Protein Variants Comment Organism
additional information expression and characterization of naturally occurring shorter forms of enzyme. Isoform GAD67 with N-terminal deletion of 70 or 90 amino acids has about 20% of wild-type activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
L-glutamate mutant with N-terminal deletion of 90 amino acids, pH 7.2 Homo sapiens
0.69
-
L-glutamate mutant with N-terminal deletion of 70 amino acids, pH 7.2 Homo sapiens
0.71
-
L-glutamate wild-type, pH 7.2 Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
recombinant isoform GAD67
-
Sus scrofa
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain both full length enzyme and a shorter form with aminoterminal deletion Sus scrofa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.039
-
mutant with N-terminal deletion of 70 amino acids, pH 7.2 Homo sapiens
0.041
-
mutant with N-terminal deletion of 90 amino acids, pH 7.2 Homo sapiens
0.216
-
wild-type, pH 7.2 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-glutamate
-
Homo sapiens 4-aminobutanoate + CO2
-
?
L-glutamate
-
Sus scrofa 4-aminobutanoate + CO2
-
?

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
Sus scrofa
pyridoxal 5'-phosphate Km-value 74 nM, wild-type, 490 nM, mutant with N-terminal deletion of 70 amino acids Homo sapiens