Literature summary for 4.1.1.15 extracted from

Papakonstantinou, T.; Law, R.H.P.; Gardiner, P.; Rowley, M.J.; Mackay, I.R.
Comparative expression and purification of human glutamic acid decarboxylase from Saccharomyces cerevisiae and Pichia pastoris (2000), Enzyme Microb. Technol., 26, 645-652.

Cloned(Commentary)

Cloned (Comment)	Organism
creation of a hybrid form of GAD consisting of amino acids 1-101 of the human GAD67 protein fused to amino acids 96-585 of the human GAD65 protein, and modification of this to include a C-terminal hexa-His tag sequence. The hybrid GAD67/65-H6 is expressed in two yeast hosts: constitutively under the control of the plasmid oxidase promoter PGK1 in Saccharomyces cerevisiae, and inducibly under the control of the chromosomal alcohol oxidase promoter AOX1 in Pichia pastoris. The hybrid GAD67/65 is isolated at high specific activity and moderate yield, and the addition of the His6 tag sequence of the choice of the yeast strain does not appreciably affect enzyme activity, percentage recovery of GAD, protein purification or utility in diagnosis of diabetes in terms of specificity and sensitivity to the various sera	Homo sapiens

Cloned (Comment)

Organism

creation of a hybrid form of GAD consisting of amino acids 1-101 of the human GAD67 protein fused to amino acids 96-585 of the human GAD65 protein, and modification of this to include a C-terminal hexa-His tag sequence. The hybrid GAD67/65-H6 is expressed in two yeast hosts: constitutively under the control of the plasmid oxidase promoter PGK1 in Saccharomyces cerevisiae, and inducibly under the control of the chromosomal alcohol oxidase promoter AOX1 in Pichia pastoris. The hybrid GAD67/65 is isolated at high specific activity and moderate yield, and the addition of the His6 tag sequence of the choice of the yeast strain does not appreciably affect enzyme activity, percentage recovery of GAD, protein purification or utility in diagnosis of diabetes in terms of specificity and sensitivity to the various sera

Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	creation of a hybrid form of GAD consisting of amino acids 1-101 of the human GAD67 protein fused to amino acids 96-585 of the human GAD65 protein, and modification of this to include a C-terminal hexa-His tag sequence. The hybrid GAD67/65-H6 is expressed in two yeast hosts: consitutively under the control of the plasmid oxidase promoter PGK1 in Saccharomyces cerevisiae, and inducibly under the control of the chromosomal alcohol oxidase promoter AOX1 in Pichia pastoris. The hybrid GAD67/65 is isolated at high specific activity and moderate yield, and the addition of the His6 tag sequence of the choice of the yeast strain does not appreciably affect enzyme activity, percentage recovery of GAD, protein purification or utility in diagnosis of diabetes in terms of specificity and sensitivity to the various sera	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.956	-	-	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-Glu	-	Homo sapiens	4-Aminobutanoate + CO2	-	?

Synonyms

Synonyms	Comment	Organism
GAD	-	Homo sapiens