Literature summary for 4.1.1.15 extracted from

Davis, K.M.; Foos, T.; Bates, C.S.; Tucker, E.; Hsu, C.C.; Chen, W.; Jin, H.; Tyburski, J.B.; Schloss, J.V.; Tobin, A.J.; Wu, J.Y.
A novel method for expression and large-scale production of human brain l-glutamate decarboxylase (2000), Biochem. Biophys. Res. Commun., 267, 777-782.

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Cloned(Commentary)

Cloned (Comment)	Organism
GST-GAD fusion protein expressed in Escherichia coli	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-Glu	Homo sapiens	rate-limiting enzyme involved in the synthesis of gamma-aminobutyric acid	4-Aminobutanoate + CO2	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
large-scale purification of GAD65 and GAD67	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1.85	-	GAD67	Homo sapiens
1.96	-	GAD65	Homo sapiens

Storage Stability

Storage Stability	Organism
-20°C, enzyme concentration of at least 3 mg/ml, under argon, in 100 mM potassium phosphate buffer containing 2 mM 2-aminoethylisothiouronium bromide and 0.4 mM pyridoxal 5'-phosphate	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-Glu	-	Homo sapiens	4-Aminobutanoate + CO2	-	?
L-Glu	rate-limiting enzyme involved in the synthesis of gamma-aminobutyric acid	Homo sapiens	4-Aminobutanoate + CO2	-	?

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Release 2023.1 (January 2023)