Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Arabidopsis sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | stimulation by Ca2+ plus calmodulin, no stimulation by Ca2+ or calmodulin alone | Arabidopsis sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
x * 56000, isoenzyme GAD2, SDS-PAGE | Arabidopsis sp. |
58000 | - |
x * 58000, isoenzyme GAD1, SDS-PAGE | Arabidopsis sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Glu | Arabidopsis sp. | isoenzyme GAD2 may play a unique role in nitrogen metabolism | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis sp. | - |
strain L. Heynh. Ecotype Columbia, 2 isoenzymes: GAD1 and GAD2 | - |
Purification (Comment) | Organism |
---|---|
recombinant isoenzymes | Arabidopsis sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-Glu | - |
Arabidopsis sp. | 4-Aminobutanoate + CO2 | - |
? | |
L-Glu | isoenzyme GAD2 may play a unique role in nitrogen metabolism | Arabidopsis sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 58000, isoenzyme GAD1, SDS-PAGE | Arabidopsis sp. |
? | x * 56000, isoenzyme GAD2, SDS-PAGE | Arabidopsis sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Calmodulin | stimulation be Ca2+ plus calmodulin, no stimulation by Ca2+ or calmodulin alone | Arabidopsis sp. |