Literature summary for 4.1.1.1 extracted from

Chen, X.; Xu, J.; Yang, L.; Yuan, Z.; Xiao, S.; Zhang, Y.; Liang, C.; He, M.; Guo, Y.
Production of C4 and C5 branched-chain alcohols by engineered Escherichia. coli (2015), J. Ind. Microbiol. Biotechnol., 42, 1473-1479.

Search for more literature for 4.1.1.1

Application

Application	Comment	Organism
synthesis	expression of branched-chain alpha-oxo acid decarboxylase from Lactococcus lactis subsp. lactis KivD and alcohol dehydrogenase from Zymomonas mobilis AdhB in Escherichia coli for higher alcohol production. In LB medium, the resulting strain produces much more 3-methyl-1-butanol (104 mg/l) than isobutanol (24 mg/l). In 5 g/l glucose-containing medium, the production of two alcohols is similar, 156 and 161 mg/l for C4 (isobutanol) and C5 (3-methyl-1-butanol) alcohol, respectively. The increase of glucose content and the adding of alpha-keto acids facilitate the production of C4 and C5 alcohols. The enzyme activities of pure Kivd on alpha-ketoisovalerate and alpha-ketoisocaproate are 26.77 and 21.24 micromol/min and mg, respectively	Lactococcus lactis subsp. lactis

Cloned(Commentary)

Cloned (Comment)	Organism
-	Lactococcus lactis subsp. lactis

Organism

Organism	UniProt	Comment	Textmining
Lactococcus lactis subsp. lactis	-	-	-
Lactococcus lactis subsp. lactis CICC 6246	-	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
21.24	-	substrate 2-oxoisocaproate, pH 6.0, 37°C	Lactococcus lactis subsp. lactis
26.77	-	substrate 2-oxoisovalerate, pH 6.0, 37°C	Lactococcus lactis subsp. lactis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2-oxoisocaproate	-	Lactococcus lactis subsp. lactis	3-methylbutanal + CO2	-	?
2-oxoisocaproate	-	Lactococcus lactis subsp. lactis CICC 6246	3-methylbutanal + CO2	-	?
2-oxoisovalerate	-	Lactococcus lactis subsp. lactis	2-methylpropanal + CO2	-	?
2-oxoisovalerate	-	Lactococcus lactis subsp. lactis CICC 6246	2-methylpropanal + CO2	-	?

Synonyms

Synonyms	Comment	Organism
KivD	-	Lactococcus lactis subsp. lactis

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Release 2023.1 (January 2023)