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Literature summary for 4.1.1.1 extracted from
- Molecular and biochemical characterization of bifunctional pyruvate decarboxylases and pyruvate ferredoxin oxidoreductases from Thermotoga maritima and Thermotoga hypogea (2015), J. Biochem., 158, 459-466.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.92 | - |
pyruvate | pH 8.4, 80°C | Thermotoga maritima |  |
| 1.4 | - |
pyruvate | pH 8.4, 80°C | Pseudothermotoga hypogea | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 13000 | - |
1 * 46000 plus 1 * 35000 plus 1 * 23000 plus 1 * 13000, SDS-PAGE | Thermotoga maritima |
| 14000 | - |
1 * 45000 plus 1 * 35000 plus 1 * 22000 plus 1 * 14000, SDS-PAGE | Pseudothermotoga hypogea |
| 22000 | - |
1 * 45000 plus 1 * 35000 plus 1 * 22000 plus 1 * 14000, SDS-PAGE | Pseudothermotoga hypogea |
| 23000 | - |
1 * 46000 plus 1 * 35000 plus 1 * 23000 plus 1 * 13000, SDS-PAGE | Thermotoga maritima |
| 35000 | - |
1 * 45000 plus 1 * 35000 plus 1 * 22000 plus 1 * 14000, SDS-PAGE | Pseudothermotoga hypogea |
| 35000 | - |
1 * 46000 plus 1 * 35000 plus 1 * 23000 plus 1 * 13000, SDS-PAGE | Thermotoga maritima |
| 45000 | - |
1 * 45000 plus 1 * 35000 plus 1 * 22000 plus 1 * 14000, SDS-PAGE | Pseudothermotoga hypogea |
| 46000 | - |
1 * 46000 plus 1 * 35000 plus 1 * 23000 plus 1 * 13000, SDS-PAGE | Thermotoga maritima |
| 156000 | - |
gel filtration | Thermotoga maritima |
| 157000 | - |
gel filtration | Pseudothermotoga hypogea |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudothermotoga hypogea | V9NGI4 and V9NFX1 and V9NGQ2 and V9NHA1 | V9NGI4, i.e. subunit PorB, V9NFX1, i.e. subunit PorD, V9NGQ2 i.e. subunit PorG, V9NHA1 i.e. subunit PorA, bifunctional pyruvate decarboxylase and pyruvate ferredoxin oxidoreductase | - |
| Thermotoga maritima | Q56317 and 05650 and Q56316 and O05651 | Q56317, i.e. subunit PorB, O05650, i.e. subunit PorC, Q56316 i.e. subunit PorD, O05651 i.e. subunit PorA, bifunctional pyruvate decarboxylase and pyruvate ferredoxin oxidoreductase | - |
| Thermotoga maritima DSM 3109 | Q56317 and 05650 and Q56316 and O05651 | Q56317, i.e. subunit PorB, O05650, i.e. subunit PorC, Q56316 i.e. subunit PorD, O05651 i.e. subunit PorA, bifunctional pyruvate decarboxylase and pyruvate ferredoxin oxidoreductase | - |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| both pyruvate decarboxylase and pyruvate ferredoxin oxidoreductase activity are extremely oxygen sensitive, 50% loss of activity upon exposure to air at room temperature within 15 min | Thermotoga maritima |
| both pyruvate decarboxylase and pyruvate ferredoxin oxidoreductase activity are extremely oxygen sensitive, 50% loss of activity upon exposure to air at room temperature within 15 min | Pseudothermotoga hypogea |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purified enzyme shos a brownish colour characteristic of iron-sulfur containing proteins | Thermotoga maritima |
| purified enzyme shows a brownish colour characteristic of iron-sulfur containing proteins | Pseudothermotoga hypogea |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.4 | - |
pH 8.4, 80°C | Thermotoga maritima |
| 1.9 | - |
pH 8.4, 80°C | Pseudothermotoga hypogea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyruvate | - |
Thermotoga maritima | acetaldehyde + CO2 | - |
? | |
| pyruvate | - |
Pseudothermotoga hypogea | acetaldehyde + CO2 | - |
? | |
| pyruvate | - |
Thermotoga maritima DSM 3109 | acetaldehyde + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 1 * 45000 plus 1 * 35000 plus 1 * 22000 plus 1 * 14000, SDS-PAGE | Pseudothermotoga hypogea |
| tetramer | 1 * 46000 plus 1 * 35000 plus 1 * 23000 plus 1 * 13000, SDS-PAGE | Thermotoga maritima |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
pyruvate decarboxylase activity | Pseudothermotoga hypogea |
| 90 | - |
pyruvate ferredoxin oxidoreductase activity | Pseudothermotoga hypogea |
| 95 | - |
above 95°C, activity is highly temperature dependent | Thermotoga maritima |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
half-life of pyruvate decarboxylase activity 2 h, of pyruvate ferredoxin oxidoreductase activity 3 h | Pseudothermotoga hypogea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.4 | - |
both pyruvate decarboxylase and pyruvate ferredoxin oxidoreductase activity | Thermotoga maritima |
| 8.4 | - |
both pyruvate decarboxylase and pyruvate ferredoxin oxidoreductase activity | Pseudothermotoga hypogea |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| CoA | Km value 0.0031 mM | Thermotoga maritima | |
| CoA | pyruvate ferredoxin oxidoreductase activity is strictly CoA-dependent, desulfo-CoA does not serve as cofactor. The pyruvate decarboxylase activity accepts desulfo-CoA, Km value for CoA is 0.0014 mM | Pseudothermotoga hypogea | |
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