Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 4.1.1.1 extracted from

  • Van Zyl, L.; Schubert, W.; Tuffin, M.; Cowan, D.
    Structure and functional characterization of pyruvate decarboxylase from Gluconacetobacter diazotrophicus (2014), BMC Struct. Biol., 14, 21.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 4.1.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Gluconacetobacter diazotrophicus

Crystallization (Commentary)

Crystallization (Comment) Organism
to 1.69 A resolution, monoclinic space group C2 with cell dimensions: a = 129.1 A, b =141.0 A, c = 91.1 A, beta = 125.8°, with two monomers per asymmetric unit Gluconacetobacter diazotrophicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
 pyruvate pH 5.0, 25°C Gluconacetobacter diazotrophicus
0.06
-
 pyruvate at pH 5.0 and 25°C Gluconacetobacter diazotrophicus
0.6
-
 pyruvate at pH 6.0 and 25°C Gluconacetobacter diazotrophicus
1.2
-
 pyruvate pH 7.0, 25°C Gluconacetobacter diazotrophicus
1.2
-
 pyruvate at pH 7.0 and 25°C Gluconacetobacter diazotrophicus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent on Gluconacetobacter diazotrophicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59200
-
-
 Gluconacetobacter diazotrophicus
59200
-
 x * 59200, calculated, x * 60000, SDS-PAGE Gluconacetobacter diazotrophicus
60000
-
 x * 60000, SDS-PAGE Gluconacetobacter diazotrophicus
60000
-
 x * 59200, calculated, x * 60000, SDS-PAGE Gluconacetobacter diazotrophicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
pyruvate Gluconacetobacter diazotrophicus
-
 acetaldehyde + CO2
-
 ?
pyruvate Gluconacetobacter diazotrophicus ATCC 49037
-
 acetaldehyde + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Gluconacetobacter diazotrophicus A0A075Q354
-
-
Gluconacetobacter diazotrophicus A9H275
-
-
Gluconacetobacter diazotrophicus ATCC 49037 A9H275
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Gluconacetobacter diazotrophicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.15
-
 substrate 2-oxo-4-methylpentanoate, pH 5.0, 25°C Gluconacetobacter diazotrophicus
0.68
-
 substrate 2-oxopentanoate, pH 5.0, 25°C Gluconacetobacter diazotrophicus
12
-
 substrate 2-oxobutanoate, pH 5.0, 25°C Gluconacetobacter diazotrophicus
20
-
 substrate pyruvate, pH 5.0, 25°C Gluconacetobacter diazotrophicus
43
-
 substrate pyruvate, pH 7.0, 25°C Gluconacetobacter diazotrophicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 pyruvate
-
 Gluconacetobacter diazotrophicus (S)-acetolactate + CO2
-
 ?
2-oxo-4-methylpentanoate
-
 Gluconacetobacter diazotrophicus 3-methylbutanaldehyde + CO2
-
 ?
2-oxo-4-methylpentanoate
-
 Gluconacetobacter diazotrophicus ?
-
 ?
2-oxo-4-methylpentanoate
-
 Gluconacetobacter diazotrophicus ATCC 49037 ?
-
 ?
2-Oxobutanoate
-
 Gluconacetobacter diazotrophicus ?
-
 ?
2-Oxobutanoate
-
 Gluconacetobacter diazotrophicus ATCC 49037 ?
-
 ?
2-oxobutanoate
-
 Gluconacetobacter diazotrophicus propionaldehyde + CO2
-
 ?
2-Oxopentanoate
-
 Gluconacetobacter diazotrophicus ?
-
 ?
2-Oxopentanoate
-
 Gluconacetobacter diazotrophicus ATCC 49037 ?
-
 ?
2-oxopentanoate
-
 Gluconacetobacter diazotrophicus butanaldehyde + CO2
-
 ?
3-phenyl-2-oxopropanoate
-
 Gluconacetobacter diazotrophicus ?
-
 ?
additional information no substrate: indole-3-pyruvate Gluconacetobacter diazotrophicus ?
-
 ?
additional information no activity with benzoylformate, 3-hydroxyphenylpyruvate and indole-3-pyruvate Gluconacetobacter diazotrophicus ?
-
 ?
additional information no activity with benzoylformate, 3-hydroxyphenylpyruvate and indole-3-pyruvate Gluconacetobacter diazotrophicus ATCC 49037 ?
-
 ?
pyruvate
-
 Gluconacetobacter diazotrophicus acetaldehyde + CO2
-
 ?
pyruvate
-
 Gluconacetobacter diazotrophicus ATCC 49037 acetaldehyde + CO2
-
 ?

Subunits

Subunits Comment Organism
? x * 60000, SDS-PAGE Gluconacetobacter diazotrophicus
? x * 59200, calculated, x * 60000, SDS-PAGE Gluconacetobacter diazotrophicus
? x * 59200, calaculated from amino acid sequence Gluconacetobacter diazotrophicus

Synonyms

Synonyms Comment Organism
PDC
-
 Gluconacetobacter diazotrophicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45 50
-
 Gluconacetobacter diazotrophicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 half-life 18 min Gluconacetobacter diazotrophicus
60
-
 the enzyme shows a half-life of18 min at 60°C Gluconacetobacter diazotrophicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5 5.5
-
 Gluconacetobacter diazotrophicus

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate dependent on Gluconacetobacter diazotrophicus

pI Value

Organism Comment pI Value Maximum pI Value
Gluconacetobacter diazotrophicus calculated
-
 5.8
Gluconacetobacter diazotrophicus calaculated from amino acid sequence
-
 5.8

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
140
-
 pyruvate pH 7.0, 25°C Gluconacetobacter diazotrophicus
140
-
 pyruvate at pH 7.0 and 25°C Gluconacetobacter diazotrophicus
260
-
 pyruvate at pH 6.0 and 25°C Gluconacetobacter diazotrophicus
1300
-
 pyruvate pH 5.0, 25°C Gluconacetobacter diazotrophicus
1300
-
 pyruvate at pH 5.0 and 25°C Gluconacetobacter diazotrophicus