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Literature summary for 4.1.1.1 extracted from

  • Cheng, M.; Yoshiyasu, H.; Okano, K.; Ohtake, H.; Honda, K.
    Redirection of the reaction specificity of a thermophilic acetolactate synthase toward acetaldehyde formation (2016), PLoS ONE, 11, e0146146.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
biotechnology the reaction specificity of acetolactate synthase from Thermus thermophilus can be redirected to catalyze acetaldehyde formation to develop a thermophilic pyruvate decarboxylase. Quadruple mutant Y35N/K139R/V172A/H474R shows 3.1fold higher acetaldehyde-forming activity than the wild-type mainly because of H474R amino acid substitution, which likely generates two new hydrogen bonds near the thiamine diphosphate-binding site Thermus thermophilus

Protein Variants

Protein Variants Comment Organism
H747R mutation leads to 3fold increased acetaldehyde formation, with 30% decrease in acetolactate formation Thermus thermophilus
Y35N/K139R/V172A/H474R shows 3.1fold higher acetaldehyde-forming activity than the wild-type Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus Q5SJ01 acetolactate synthase, EC 2.2.1.6
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Thermus thermophilus HB8 / ATCC 27634 / DSM 579 Q5SJ01 acetolactate synthase, EC 2.2.1.6
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.45
-
wild-type, pH 7.0, 60°C Thermus thermophilus
4.51
-
mutant Y35N/K139R/V172A/H474R, pH 7.0, 60°C Thermus thermophilus
5.52
-
mutant H747R, pH 7.0, 60°C Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pyruvate
-
Thermus thermophilus acetaldehyde + CO2 in wild-type, about 10% of the acetolactate forming activity ?
pyruvate
-
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 acetaldehyde + CO2 in wild-type, about 10% of the acetolactate forming activity ?

Synonyms

Synonyms Comment Organism
TTHA1213
-
Thermus thermophilus

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate
-
Thermus thermophilus