Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 18 mM citrate/2 mM MES, pH 6.3, 2 mM dithiothreitol, 2 mM thiamine diphosphate, 2 mM MgSO4, 22.5% (w/v) PEG 2000/PEG 6000 (1:1 ratio) | Saccharomyces cerevisiae |
hanging drop vapor diffusion method, using 20 mM citrate buffer, pH 6.1, 1 mM dithiothreitol, 5 mM thiamine diphosphate, 5 mM MgSO4, 20% (w/v) PEG 2000/PEG 6000 (1:1 ratio) | Kluyveromyces lactis |
Protein Variants | Comment | Organism |
---|---|---|
D28A | the mutant is almost catalytically inactive | Kluyveromyces lactis |
D28A | the mutant is almost catalytically inactive | Saccharomyces cerevisiae |
E477Q | the mutant is almost catalytically inactive | Kluyveromyces lactis |
E477Q | the mutant is almost catalytically inactive | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Kluyveromyces lactis | - |
- |
- |
Saccharomyces cerevisiae | P06169 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Kluyveromyces lactis |
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate | - |
Kluyveromyces lactis | acetaldehyde + CO2 | - |
? | |
pyruvate | - |
Saccharomyces cerevisiae | acetaldehyde + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PDC | - |
Kluyveromyces lactis |
PDC | - |
Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | - |
Kluyveromyces lactis | |
thiamine diphosphate | - |
Saccharomyces cerevisiae |