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Literature summary for 4.1.1.1 extracted from

  • Yep, A.; Kenyon, G.L.; McLeish, M.J.
    Determinants of substrate specificity in KdcA, a thiamin diphosphate-dependent decarboxylase (2006), Bioorg. Chem., 34, 325-336.
    View publication on PubMed
Search for more literature for 4.1.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expression of enzyme mutants in Escherichia coli strain BL21(DE3) Lactococcus lactis

Protein Variants

Protein Variants Comment Organism
F381W site-directed mutagenesis, mutation of KdcA, a branched chain 2-keto acid decarboxylase, EC 4.1.1.72, alters the substrate specificity to a pyruvate decarboxylase showing high kcat and activity with pyruvate compared to the wild-type enzyme Lactococcus lactis
M538W site-directed mutagenesis, mutation of KdcA, a branched chain 2-keto acid decarboxylase, EC 4.1.1.72, alters the substrate specificity to a pyruvate decarboxylase showing higher kcat and activity with pyruvate compared to the wild-type enzyme Lactococcus lactis
S286Y site-directed mutagenesis, mutation of KdcA, a branched chain 2-keto acid decarboxylase, EC 4.1.1.72, alters the substrate specificity to a pyruvate decarboxylase showing high kcat and activity with pyruvate compared to the wild-type enzyme Lactococcus lactis
V461I site-directed mutagenesis, mutation of KdcA, a branched chain 2-keto acid decarboxylase, EC 4.1.1.72, alters the substrate specificity to a pyruvate decarboxylase showing higher kcat and activity with pyruvate compared to the wild-type enzyme Lactococcus lactis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics analysis of the wild-type enzyme with beta-hydroxypyruvate as substrate in the decarboxylation reaction Lactococcus lactis
18
-
 Pyruvic acid pH 6.0, 30°C, recombinant mutant V461I Lactococcus lactis
22
-
 Pyruvic acid pH 6.0, 30°C, recombinant mutant M538W Lactococcus lactis
34
-
 Pyruvic acid pH 6.0, 30°C, recombinant mutant S286Y Lactococcus lactis
65
-
 Pyruvic acid pH 6.0, 30°C, recombinant mutant F381W Lactococcus lactis

Organism

Organism UniProt Comment Textmining
Lactococcus lactis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme mutants from Escherichia coli strain BL21(DE3) Lactococcus lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxohexanoic acid the structural basis for KdcA, a branched chain 2-keto acid decarboxylase, EC 4.1.1.72, substrate recognition involving residues Ser286, Phe381, Val461 and Met358 of the substrate binding pocket, mutation of Ser286 and Phe381 converts the enzyme to a pyruvate decarboxylase, homology modeling, overview Lactococcus lactis pentanal + CO2
-
 ?
2-oxopentanoic acid the structural basis for KdcA, a branched chain 2-keto acid decarboxylase, EC 4.1.1.72, substrate recognition involving residues Ser286, Phe381, Val461 and Met358 of the substrate binding pocket, mutation of Ser286 and Phe381 converts the enzyme to a pyruvate decarboxylase, homology modeling, overview Lactococcus lactis butanal + CO2
-
 ?
additional information substrate specificity of the engineered KdcA mutant enzymes with branched and unbranched 2-oxo acid substrates, overview Lactococcus lactis ?
-
 ?
pyruvic acid the structural basis for KdcA, a branched chain 2-keto acid decarboxylase, EC 4.1.1.72, substrate recognition involving residues Ser286, Phe381, Val461 and Met358 of the substrate binding pocket, mutation of Ser286 and Phe381 converts the enzyme to a pyruvate decarboxylase, homology modeling, overview Lactococcus lactis acetaldehyde + CO2
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Lactococcus lactis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3
-
 Pyruvic acid pH 6.0, 30C, recombinant mutant V461I Lactococcus lactis
2.5
-
 Pyruvic acid pH 6.0, 30C, recombinant mutant M538W Lactococcus lactis
17
-
 Pyruvic acid pH 6.0, 30C, recombinant mutant S286Y Lactococcus lactis
26
-
 Pyruvic acid pH 6.0, 30C, recombinant mutant F381W Lactococcus lactis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Lactococcus lactis

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate dependent on Lactococcus lactis