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Literature summary for 4.1.1.1 extracted from

  • Siegert, P.; McLeish, M.J.; Baumann, M.; Iding, H.; Kneen, M.M.; Kenyon, G.L.; Pohl, M.
    Exchanging the substrate specificities of pyruvate decarboxylase from Zymomonas mobilis and benzoylformate decarboxylase from Pseudomonas putida (2005), Protein Eng. Des. Sel., 18, 345-357.
    View publication on PubMed
Search for more literature for 4.1.1.1

Protein Variants

Protein Variants Comment Organism
I472A/I476F increase in substrate binding affinity and specificity, highest enantioselectivity for (S)-acetoin, very low yield of product Zymomonas mobilis
I476F rapid loss of cofactor thiamine diphosphate. Improvement of enantioselectivity for (S)-acetoin Zymomonas mobilis
mutant I472A 2fold decrease in pyruvate decarboxylase activity, switch in substrate specificity to catalyse decarboxylation of benzoylformate, chimera between pyruvate decarboxylase and benzoylformate decarboxylase. Preferred substrates are 2-ketopentanoic acid and 2-ketohexanoic acid. Improvement of enantioselectivity for (S)-acetoin Zymomonas mobilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 2-ketohexanoic acid mutant I472A, pH 6.5, 30°C Zymomonas mobilis
0.5
-
 2-ketohexanoic acid mutant I472A/I476F, pH 6.5, 30°C Zymomonas mobilis
1.1
-
 pyruvate wild type, pH 6.5, 30°C Zymomonas mobilis
1.7
-
 2-keto-4-methylhexanoic acid mutant I472A, pH 6.5, 30°C Zymomonas mobilis
1.8
-
 benzoylformate mutant I472A, pH 6.5, 30°C Zymomonas mobilis
2.5
-
 2-ketopentanoic acid mutant I472A, pH 6.5, 30°C Zymomonas mobilis
2.6
-
 pyruvate mutant I476F, pH 6.5, 30°C Zymomonas mobilis
3.7
-
 2-keto-4-methylhexanoic acid mutant I472A/I476F, pH 6.5, 30°C Zymomonas mobilis
4.4
-
 benzoylformate mutant I472A/I476F, pH 6.5, 30°C Zymomonas mobilis
4.7
-
 2-ketobutanoic acid wild type, pH 6.5, 30°C Zymomonas mobilis
6.7
-
 2-ketobutanoic acid mutant I472A, pH 6.5, 30°C Zymomonas mobilis
7.6
-
 2-ketopentanoic acid wild type, pH 6.5, 30°C Zymomonas mobilis
7.8
-
 pyruvate mutant I472A, pH 6.5, 30°C Zymomonas mobilis
11
-
 2-ketopentanoic acid mutant I472A/I476F, pH 6.5, 30°C Zymomonas mobilis
12.7
-
 2-ketohexanoic acid wild type, pH 6.5, 30°C Zymomonas mobilis
40
-
 2-keto-4-methylhexanoic acid and above, wild type, pH 6.5, 30°C Zymomonas mobilis
50
-
 pyruvate mutant I472A/I476F, pH 6.5, 30°C Zymomonas mobilis
50
-
 2-ketobutanoic acid mutant I472A/I476F, pH 6.5, 30°C Zymomonas mobilis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Zymomonas mobilis

Organism

Organism UniProt Comment Textmining
Zymomonas mobilis P06672
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-keto-4-methylhexanoic acid
-
 Zymomonas mobilis 3-methylpentanal + CO2
-
 ?
2-ketobutanoic acid
-
 Zymomonas mobilis propanal + CO2
-
 ?
2-ketohexanoic acid
-
 Zymomonas mobilis pentanal + CO2
-
 ?
2-ketopentanoic acid
-
 Zymomonas mobilis butanal + CO2
-
 ?
benzoylformate substrate only for mutant mutant I472A Zymomonas mobilis benzaldehyde + CO2
-
 ?
pyruvate
-
 Zymomonas mobilis acetaldehyde + CO2
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.4
-
 2-keto-4-methylhexanoic acid wild type, pH 6.5, 30°C Zymomonas mobilis
0.8
-
 2-ketohexanoic acid mutant I476F, pH 6.5, 30°C Zymomonas mobilis
1.2
-
 benzoylformate mutant I472A/I476F, pH 6.5, 30°C Zymomonas mobilis
4
-
 2-ketohexanoic acid wild type, pH 6.5, 30°C Zymomonas mobilis
6.9
-
 benzoylformate mutant I472A, pH 6.5, 30°C Zymomonas mobilis
7.7
-
 2-keto-4-methylhexanoic acid mutant I472A, pH 6.5, 30°C Zymomonas mobilis
8
-
 pyruvate mutant I472A/I476F, pH 6.5, 30°C Zymomonas mobilis
8.1
-
 2-keto-4-methylhexanoic acid mutant I472A/I476F, pH 6.5, 30°C Zymomonas mobilis
9
-
 2-ketobutanoic acid mutant I472A/I476F, pH 6.5, 30°C Zymomonas mobilis
11
-
 2-ketopentanoic acid mutant I476F, pH 6.5, 30°C Zymomonas mobilis
30
-
 2-ketopentanoic acid mutant I472A/I476F, pH 6.5, 30°C Zymomonas mobilis
32
-
 2-ketobutanoic acid mutant I476F, pH 6.5, 30°C Zymomonas mobilis
53
-
 2-ketopentanoic acid wild type, pH 6.5, 30°C Zymomonas mobilis
77
-
 pyruvate mutant I476F, pH 6.5, 30°C Zymomonas mobilis
82
-
 2-ketohexanoic acid mutant I472A/I476F, pH 6.5, 30°C Zymomonas mobilis
130
-
 2-ketohexanoic acid mutant I472A, pH 6.5, 30°C Zymomonas mobilis
200
-
 pyruvate mutant I472A, pH 6.5, 30°C Zymomonas mobilis
220
-
 2-ketopentanoic acid mutant I472A, pH 6.5, 30°C Zymomonas mobilis
250
-
 2-ketobutanoic acid mutant I472A, pH 6.5, 30°C Zymomonas mobilis
320
-
 2-ketobutanoic acid wild type, pH 6.5, 30°C Zymomonas mobilis
486
-
 pyruvate wild type, pH 6.5, 30°C Zymomonas mobilis

Cofactor

Cofactor Comment Organism Structure
thiamine diphosphate
-
 Zymomonas mobilis