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Literature summary for 4.1.1.1 extracted from
- Production of the gram-positive Sarcina ventriculi pyruvate decarboxylase in Escherichia coli (2001), Microbiology, 147, 2425-2435.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| pyruvate | substrate activator is allosterically bound to enzyme, mechanism | Sarcina ventriculi |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| pdc gene, sequencing, expression in Escherichia coli BL-21-CodonPlus-RIL containing plasmid pSJS1240 | Sarcina ventriculi |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| maleate buffer | inhibits at low pyruvate concentrations | Sarcina ventriculi | |
| additional information | not inhibited by 25 mM EGTA or EDTA, at 37°C, pH 6.5, 90 min, without cofactors | Sarcina ventriculi |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.8 | - |
pyruvate | pH 6.5, potassium MES buffer, two affinities for pyruvate, sigmoidal kinetics | Sarcina ventriculi | |
| 10 | - |
pyruvate | pH 6.5, sodium hydrogen maleate buffer, two affinities for pyruvate, sigmoidal kinetics | Sarcina ventriculi | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | recombinant PDC, expressed in Escherichia coli | Sarcina ventriculi | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | cofactor, bound with high affinity at slightly acidic pH | Sarcina ventriculi | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
4 * 58000, recombinant PDC, pH 6.5, SDS-PAGE | Sarcina ventriculi |
| 235000 | - |
recombinant PDC, pH 6.5, gel filtration | Sarcina ventriculi |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyruvate | Sarcina ventriculi | during growth in acidic environments, where acetate is toxic, expression of PDC serves to direct the flow of pyruvate into ethanol during fermentation | acetaldehyde + CO2 | - |
? | |
| pyruvate | Sarcina ventriculi Goodsir / ATCC 55887 | during growth in acidic environments, where acetate is toxic, expression of PDC serves to direct the flow of pyruvate into ethanol during fermentation | acetaldehyde + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sarcina ventriculi | Q93EN4 | - |
- |
| Sarcina ventriculi Goodsir / ATCC 55887 | Q93EN4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 136fold, recombinant PDC, expressed in Escherichia coli | Sarcina ventriculi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyruvate | nonoxidative decarboxylation | Sarcina ventriculi | acetaldehyde + CO2 | - |
? | |
| pyruvate | during growth in acidic environments, where acetate is toxic, expression of PDC serves to direct the flow of pyruvate into ethanol during fermentation | Sarcina ventriculi | acetaldehyde + CO2 | - |
? | |
| pyruvate | nonoxidative decarboxylation | Sarcina ventriculi Goodsir / ATCC 55887 | acetaldehyde + CO2 | - |
? | |
| pyruvate | during growth in acidic environments, where acetate is toxic, expression of PDC serves to direct the flow of pyruvate into ethanol during fermentation | Sarcina ventriculi Goodsir / ATCC 55887 | acetaldehyde + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homotetramer | 4 * 58000, recombinant PDC, pH 6.5, SDS-PAGE | Sarcina ventriculi |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PDC | - |
Sarcina ventriculi |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 42 | - |
in presence of 1 mM thiamine diphosphate, 1 mM Mg2+, pH 6.5, stable up to | Sarcina ventriculi |
| 50 | - |
60-90 min, complete loss of activity | Sarcina ventriculi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.3 | 6.7 | - |
Sarcina ventriculi |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| thiamine diphosphate | bound with high affinity at slightly acidic pH | Sarcina ventriculi | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Sarcina ventriculi | calculated from the sequence | - |
5.2 |
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