Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Pyruvamide | artificial activator | Kluyveromyces lactis | |
pyruvate | concentration-dependent substrate activation, minimum at 1.5 mM, mechanism, kinetics | Kluyveromyces lactis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Pyruvamide | mixed type inhibitor | Kluyveromyces lactis | |
pyruvate | weak substrate inhibition, above 100 mM | Kluyveromyces lactis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic studies | Kluyveromyces lactis | |
0.24 | - |
pyruvate | pH 6, 30°C | Kluyveromyces lactis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Kluyveromyces lactis | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | cofactor, requirement | Kluyveromyces lactis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61500 | - |
4 * 61500, mass spectrometry, SDS-PAGE, 4 * 61821, calculated from the amino acid sequence | Kluyveromyces lactis |
61821 | - |
4 * 61500, mass spectrometry, SDS-PAGE, 4 * 61821, calculated from the amino acid sequence | Kluyveromyces lactis |
200000 | - |
about, gel filtration | Kluyveromyces lactis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate | Kluyveromyces lactis | key enzyme at the branching point of alcoholic fermentation and respiration, expression at high glucose and low oxygen concentration | acetaldehyde + CO2 | - |
? | |
pyruvate | Kluyveromyces lactis JA-6 | key enzyme at the branching point of alcoholic fermentation and respiration, expression at high glucose and low oxygen concentration | acetaldehyde + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Kluyveromyces lactis | - |
- |
- |
Kluyveromyces lactis JA-6 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Kluyveromyces lactis |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a 2-oxo carboxylate = an aldehyde + CO2 | catalytic mechanism | Kluyveromyces lactis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Kluyveromyces lactis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate | catalytic mechanism, contains catalytic and regulatory pyruvate binding site | Kluyveromyces lactis | acetaldehyde + CO2 | - |
? | |
pyruvate | key enzyme at the branching point of alcoholic fermentation and respiration, expression at high glucose and low oxygen concentration | Kluyveromyces lactis | acetaldehyde + CO2 | - |
? | |
pyruvate | catalytic mechanism, contains catalytic and regulatory pyruvate binding site | Kluyveromyces lactis JA-6 | acetaldehyde + CO2 | - |
? | |
pyruvate | key enzyme at the branching point of alcoholic fermentation and respiration, expression at high glucose and low oxygen concentration | Kluyveromyces lactis JA-6 | acetaldehyde + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 61500, mass spectrometry, SDS-PAGE, 4 * 61821, calculated from the amino acid sequence | Kluyveromyces lactis |
Synonyms | Comment | Organism |
---|---|---|
KlPDC | - |
Kluyveromyces lactis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Kluyveromyces lactis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
40 | - |
pyruvate | pH 6, 30°C, enzyme monomer | Kluyveromyces lactis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Kluyveromyces lactis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | requirement | Kluyveromyces lactis |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
1200 | - |
pyruvate | pH 6, 30°C | Kluyveromyces lactis |