Activating Compound | Comment | Organism | Structure |
---|---|---|---|
pyruvate | substrate activation | Zygosaccharomyces bisporus |
Cloned (Comment) | Organism |
---|---|
cDNA sequence | Zygosaccharomyces bisporus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phenylpyruvate | 10 mM, 81% inhibition | Zygosaccharomyces bisporus | |
pyruvate | substrate inhibition above 25 mM | Zygosaccharomyces bisporus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.73 | - |
pyruvate | pH 6, 25°C, sigmoidal dependence of the reaction rate from substrate concentration, Hill coefficient 2.10 | Zygosaccharomyces bisporus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | cofactor | Zygosaccharomyces bisporus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61000 | - |
alpha4, 4 * 61000, SDS-PAGE, 4 * 61600, amino acid sequence | Zygosaccharomyces bisporus |
61600 | - |
alpha4, 4 * 61000, SDS-PAGE, 4 * 61600, amino acid sequence | Zygosaccharomyces bisporus |
244000 | - |
active enzyme, gel filtration | Zygosaccharomyces bisporus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate | Zygosaccharomyces bisporus | key enzyme in alcoholic fermentation | acetaldehyde + CO2 | - |
? | |
pyruvate | Zygosaccharomyces bisporus CBS 702 | key enzyme in alcoholic fermentation | acetaldehyde + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zygosaccharomyces bisporus | Q9UUT6 | - |
- |
Zygosaccharomyces bisporus CBS 702 | Q9UUT6 | - |
- |
Purification (Comment) | Organism |
---|---|
41fold | Zygosaccharomyces bisporus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Zygosaccharomyces bisporus |
Storage Stability | Organism |
---|---|
-20°C, concentrated PDC, buffered solution containing thiamine diphosphate and MgSO4, several months, stable | Zygosaccharomyces bisporus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | nonoxidative decarboxylation of pyruvate and other 2-oxo-acids | Zygosaccharomyces bisporus | ? | - |
? | |
additional information | nonoxidative decarboxylation of pyruvate and other 2-oxo-acids | Zygosaccharomyces bisporus CBS 702 | ? | - |
? | |
Phenylpyruvate | - |
Zygosaccharomyces bisporus | Phenylacetaldehyde + CO2 | - |
? | |
Phenylpyruvate | - |
Zygosaccharomyces bisporus CBS 702 | Phenylacetaldehyde + CO2 | - |
? | |
phenylpyruvate + acetaldehyde | enzyme catalyzes a carboligation as side reaction | Zygosaccharomyces bisporus | 3-hydroxy-1-phenyl-butan-2-one + CO2 | - |
? | |
phenylpyruvate + acetaldehyde | enzyme catalyzes a carboligation as side reaction | Zygosaccharomyces bisporus CBS 702 | 3-hydroxy-1-phenyl-butan-2-one + CO2 | - |
? | |
pyruvate | - |
Zygosaccharomyces bisporus | acetaldehyde + CO2 | - |
? | |
pyruvate | key enzyme in alcoholic fermentation | Zygosaccharomyces bisporus | acetaldehyde + CO2 | - |
? | |
pyruvate | - |
Zygosaccharomyces bisporus CBS 702 | acetaldehyde + CO2 | - |
? | |
pyruvate | key enzyme in alcoholic fermentation | Zygosaccharomyces bisporus CBS 702 | acetaldehyde + CO2 | - |
? | |
pyruvate + phenylacetaldehyde | enzyme catalyzes a carboligation as side reaction | Zygosaccharomyces bisporus | 3-hydroxy-4-phenyl-butan-2-one + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | alpha4, 4 * 61000, SDS-PAGE, 4 * 61600, amino acid sequence | Zygosaccharomyces bisporus |
Synonyms | Comment | Organism |
---|---|---|
ZbPDC | - |
Zygosaccharomyces bisporus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Zygosaccharomyces bisporus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Zygosaccharomyces bisporus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | - |
Zygosaccharomyces bisporus |