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Literature summary for 4.1.1.1 extracted from
- Solvent kinetic isotope effects monitor changes in hydrogen bonding at the active center of yeast pyruvate decarboxylase concomitant with substrate activation: the substituent at position 221 can control the state of activation (2002), Biochemistry, 41, 451-461.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Pyruvamide | activates | Saccharomyces cerevisiae |  |
| pyruvate | substrate activation, interaction of pyruvate with residue C221 provides the trigger, transmitting the information along the C221 to H92 to E91 to W412 to G413 pathway to the 4-amino nitrogen of the thiamine diphosphate cofactor, changes in hydrogen bonding at the active center as a result of substrate activation, mechanism | Saccharomyces cerevisiae | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL21(DE3) | Saccharomyces cerevisiae |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C221A | active mutant with reduced Hill coefficient of 1 | Saccharomyces cerevisiae |
| C221A/C222A | active double mutant, effect on transition states | Saccharomyces cerevisiae |
| C221D/C222A | double mutant with 70% of wild-type activity, but reduced Hill coefficient of 1, no substrate activation, effect on transition states, kinetics | Saccharomyces cerevisiae |
| C221E/C222A | double mutant with 70% of wild-type activity, but reduced Hill coefficient of 1, no substrate activation, effect on transition states, kinetics | Saccharomyces cerevisiae |
| C221S | active mutant with reduced Hill coefficient of 0.8-0.9 | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Pyruvamide | inhibits at high concentrations | Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | pH-dependent kinetic data of wild-type, C221E/C222A and C221A/C222A double mutant YPDC | Saccharomyces cerevisiae |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
x * 60000, about, SDS-PAGE, x * 61486, calculated from the amino acid sequence | Saccharomyces cerevisiae |
| 61486 | - |
x * 60000, about, SDS-PAGE, x * 61486, calculated from the amino acid sequence | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyruvate | Saccharomyces cerevisiae | - |
acetaldehyde + CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| wild-type, C221E/C222A and C221A/C222A double mutant YPDC | Saccharomyces cerevisiae |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| a 2-oxo carboxylate = an aldehyde + CO2 | mechanism | Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| pyruvate | - |
Saccharomyces cerevisiae | acetaldehyde + CO2 | - |
? | |
| pyruvate | mechanism | Saccharomyces cerevisiae | acetaldehyde + CO2 | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 60000, about, SDS-PAGE, x * 61486, calculated from the amino acid sequence | Saccharomyces cerevisiae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| YPDC | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Saccharomyces cerevisiae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kcat values of wild-type, C221E/C222A and C221A/C222A double mutant YPDC at different pH values between pH 5 and 7.2 | Saccharomyces cerevisiae | |
| 0.99 | - |
pyruvate | pH 6, 25°C, C221E/C222A double mutant YPDC | Saccharomyces cerevisiae | |
| 3.8 | - |
pyruvate | pH 6, 25°C, C221D/C222A double mutant YPDC | Saccharomyces cerevisiae | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| thiamine diphosphate | requirement, active center, at the interface of the alpha and gamma domains | Saccharomyces cerevisiae | |
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