Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Pyruvamide | activation mechanism, binds only at the regulatory site, but with lower affinity than does pyruvate | Saccharomyces cerevisiae | |
pyruvate | allosteric substrate activation, activation mechanism | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
C221A | mutant lacking the binding site for the regulatory pyruvate molecule with 25% of wild-type activity at pH 6 | Saccharomyces cerevisiae |
C221D | mutant with nearly wild-type activity, hyperbolic kinetics | Saccharomyces cerevisiae |
C221E | mutant with nearly wild-type activity, hyperbolic kinetics | Saccharomyces cerevisiae |
C221S | mutant lacking the binding site for the regulatory pyruvate molecule with 25% of wild-type activity at pH 6 | Saccharomyces cerevisiae |
D28A | active site mutant, kinetic properties, effect of the mutation on the activation/inhibition properties of pyruvate | Saccharomyces cerevisiae |
D28N | active site mutant, kinetic properties, effect of the mutation on the activation/inhibition properties of pyruvate | Saccharomyces cerevisiae |
E477Q | active site mutant, kinetic properties, effect of the mutation on the activation/inhibition properties of pyruvate | Saccharomyces cerevisiae |
E51D | mutant with 50% of wild-type acetaldehyde producing activity | Saccharomyces cerevisiae |
H114F | active site mutant | Saccharomyces cerevisiae |
H115F | active site mutant | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
pyruvate | modest substrate inhibition at high concentrations | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic model, kinetic data | Saccharomyces cerevisiae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | cofactor, requirement | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
pyruvate | Saccharomyces cerevisiae | - |
acetaldehyde + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a 2-oxo carboxylate = an aldehyde + CO2 | mechanism, catalyzes carboligation as side reaction | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme catalyzes also a carboligation as side reaction producing acetoin and acetolactate, mechanism, not: pyruvamide | Saccharomyces cerevisiae | ? | - |
? | |
pyruvate | - |
Saccharomyces cerevisiae | acetaldehyde + CO2 | - |
? | |
pyruvate | detailed mechanism with roles for the active center acid-base groups D28, E477, H114 and H115, catalytic cycle, mechanistic model of the reaction, alternating sites model | Saccharomyces cerevisiae | acetaldehyde + CO2 | - |
ir |
Subunits | Comment | Organism |
---|---|---|
homotetramer | dimer of dimers, the minimal catalytic unit is the dimer with its active sites are not acting independently of one another, alternating sites model | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
YPDC | - |
Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.8 | 6 | wild-type YPDC | Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7.2 | nearly identical Vmax values in the pH range, wild-type YPDC | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
thiamine diphosphate | requirement, bound at the interface created by 2 subunits that form a tight dimer, mode of binding | Saccharomyces cerevisiae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Saccharomyces cerevisiae | |
3.75 | - |
pyruvate | D28A mutant YPDC | Saccharomyces cerevisiae | |
112.2 | - |
pyruvate | pH 6, E477Q mutant YPDC | Saccharomyces cerevisiae |