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Literature summary for 4.1.1.1 extracted from
- Purification and characterisation of pyruvate decarboxylase from pea seeds (Pisum sativum cv. Miko) (1995), Biol. Chem. Hoppe-Seyler, 376, 111-117.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Pyruvamide | activates | Pisum sativum |  |
General Stability
| General Stability | Organism |
|---|---|
| Mg2+ and thiamin diphosphate stabilize | Pisum sativum |
| not sensitive to low ionic strength, 10 mM buffer | Pisum sativum |
| not stabilized by addition of ammonium sulfate | Pisum sativum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Pisum sativum | key enzyme in ethanol formation | ? | - |
? | |
| additional information | Pisum sativum | key enzyme in alcoholic fermentation | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pisum sativum | - |
cv. Miko | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pisum sativum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Pisum sativum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Pisum sativum |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C or -80°C, pH 6.0, 1 mM thiamine diphosphate, 1 mM MgSO4, minimum protein concentration 0.2 mg/ml, stable for several months | Pisum sativum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | key enzyme in ethanol formation | Pisum sativum | ? | - |
? | |
| additional information | key enzyme in alcoholic fermentation | Pisum sativum | ? | - |
? | |
| pyruvate | - |
Pisum sativum | acetaldehyde + CO2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | 2 protein bands detected in SDS-PAGE: 65000 and 68000, the enzyme exists as a mixture of tetramers, octamers and higher oligomers | Pisum sativum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
maximal stability | Pisum sativum |
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