Application | Comment | Organism |
---|---|---|
additional information | the L-haloacid dehalogenase from a psychrotrophic Pseudoalteromonas from the Arctic revealed its potential in industrial applications such as in synthetic chemistry and environmental protection | Pseudoalteromonas sp. |
Cloned (Comment) | Organism |
---|---|
phylogenetic analysis, recombinant expression of N-terminally His- and S-tagged enzyme in Escherichia coli strain BMRosetta | Pseudoalteromonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
monobromoacetic acid | pH 8.2, 25°C | Pseudoalteromonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
27800 | - |
x * 27800, about, sequence calculation, x * 25600-35600, recombinant tagged enzyme, SDS-PAGE, x * 32500, about, recombinant tagged enzyme, sequence calculation | Pseudoalteromonas sp. |
32500 | - |
x * 27800, about, sequence calculation, x * 25600-35600, recombinant tagged enzyme, SDS-PAGE, x * 32500, about, recombinant tagged enzyme, sequence calculation | Pseudoalteromonas sp. |
Organic Solvent | Comment | Organism |
---|---|---|
acetonitrile | 20-40% of activity remains after incubation in 40% of DMSO | Pseudoalteromonas sp. |
DMSO | about 92% of activity remains after incubation in 40% of DMSO | Pseudoalteromonas sp. |
Ethanol | 20-40% of activity remains after incubation in 40% of DMSO | Pseudoalteromonas sp. |
Methanol | 20-40% of activity remains after incubation in 40% of DMSO | Pseudoalteromonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudoalteromonas sp. | - |
isolated from the Arctic marine environment | - |
Pseudoalteromonas sp. BSW20308 | - |
isolated from the Arctic marine environment | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His- and S-tagged enzyme from Escherichia coli strain BMRosetta by affinity purification followed by gel filtration, to homogeneity | Pseudoalteromonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-2-bromopropionic acid + H2O | 41% activity compared to monobromoacetic acid | Pseudoalteromonas sp. | (R)-2-hydroxypropionic acid + bromide | - |
? | |
(S)-2-bromopropionic acid + H2O | 41% activity compared to monobromoacetic acid | Pseudoalteromonas sp. BSW20308 | (R)-2-hydroxypropionic acid + bromide | - |
? | |
(S)-2-chlorobutyric acid + H2O | below 10% activity compared to monobromoacetic acid | Pseudoalteromonas sp. | (R)-2-hydroxybutyric acid + chloride | - |
? | |
(S)-2-chlorobutyric acid + H2O | below 10% activity compared to monobromoacetic acid | Pseudoalteromonas sp. BSW20308 | (R)-2-hydroxybutyric acid + chloride | - |
? | |
(S)-2-chloropropionic acid + H2O | 14% activity compared to monobromoacetic acid | Pseudoalteromonas sp. | (R)-2-hydroxypropionic acid + chloride | - |
? | |
(S)-2-chloropropionic acid + H2O | 14% activity compared to monobromoacetic acid | Pseudoalteromonas sp. BSW20308 | (R)-2-hydroxypropionic acid + chloride | - |
? | |
dichloroacetic acid + H2O | below 10% activity compared to monobromoacetic acid | Pseudoalteromonas sp. | ? | - |
? | |
dichloroacetic acid + H2O | below 10% activity compared to monobromoacetic acid | Pseudoalteromonas sp. BSW20308 | ? | - |
? | |
monobromoacetic acid + H2O | best substrate | Pseudoalteromonas sp. | acetic acid + bromide | - |
? | |
monobromoacetic acid + H2O | best substrate | Pseudoalteromonas sp. BSW20308 | acetic acid + bromide | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 27800, about, sequence calculation, x * 25600-35600, recombinant tagged enzyme, SDS-PAGE, x * 32500, about, recombinant tagged enzyme, sequence calculation | Pseudoalteromonas sp. |
Synonyms | Comment | Organism |
---|---|---|
2-HAD | - |
Pseudoalteromonas sp. |
2-haloacid dehalogenase | - |
Pseudoalteromonas sp. |
2-haloalkanoate dehalogenase | - |
Pseudoalteromonas sp. |
HADIIBSW | - |
Pseudoalteromonas sp. |
L-HAD | - |
Pseudoalteromonas sp. |
L-haloacid dehalogenase | - |
Pseudoalteromonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Pseudoalteromonas sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 65 | activity range, 20% of maximal activity at 10°C, about 80% at 60°C, inactivation above 65°C | Pseudoalteromonas sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
purified recombinant enzyme, 2 h, completely stable up to | Pseudoalteromonas sp. |
60 | - |
purified recombinant enzyme, 2 h, 40% activity remaining | Pseudoalteromonas sp. |
65 | - |
purified recombinant enzyme, 2 h, inactivation | Pseudoalteromonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
assay at | Pseudoalteromonas sp. |
General Information | Comment | Organism |
---|---|---|
evolution | 2-HADs can be classified into two phylogenetically distinct groups according to their substrate specificity. Group II consists of only L-HADs, which selectively act on L-2-haloalkanoic acids to produce D-2-hydroxyacids. HADIIBSW is evolutionarily distinct from previously characterized L-HADs, sharing only 34% identity with L-HAD (DehRhb) from a Rhodobacteraceae family bacterium UDC319 as the closest relative | Pseudoalteromonas sp. |