Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-Codon Plus (DE3)-RIL cells | Pyrococcus horikoshii |
Crystallization (Comment) | Organism |
---|---|
oil-microbatch method, in 200 mM NaCl and 20 mM Tris-HCl pH 8.0, 25.5% (w/v) polyethylene glycol 4000, 0.17 M ammonium acetate, 15% (w/v) glycerol and 85 mM sodium acetate buffer, pH 4.6, at 20°C | Pyrococcus horikoshii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | dependent on | Pyrococcus horikoshii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25600 | - |
SDS-PAGE | Pyrococcus horikoshii |
49700 | - |
calculated apparent molecular weight | Pyrococcus horikoshii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii | - |
strain OT3 | - |
Pyrococcus horikoshii OT-3 | - |
strain OT3 | - |
Purification (Comment) | Organism |
---|---|
HiPrep 26/10 column chromatography, Super Q Toyopearl 650M column chromatography, hydroxyapatite column chromatography, and Superdex 200 gel filtration | Pyrococcus horikoshii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | PH1421 is a magnesium-dependent haloacid dehalogenase phosphatase that acts on compounds closely related to 2-phosphoglycolate, the enzyme does possess 4-nitrophenyl phosphate hydrolase activity in the presence of Mg2+ | Pyrococcus horikoshii | ? | - |
? | |
additional information | PH1421 is a magnesium-dependent haloacid dehalogenase phosphatase that acts on compounds closely related to 2-phosphoglycolate, the enzyme does possess 4-nitrophenyl phosphate hydrolase activity in the presence of Mg2+ | Pyrococcus horikoshii OT-3 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 25600, SDS-PAGE | Pyrococcus horikoshii |
Synonyms | Comment | Organism |
---|---|---|
L-2-haloacid dehalogenase | - |
Pyrococcus horikoshii |
PH1421 | - |
Pyrococcus horikoshii |