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Literature summary for 3.8.1.2 extracted from

  • Liu, J.Q.; Kurihara, T.; Nardi-Dei, V.; Okamura, T.; Esaki, N.; Soda, K.
    Overexpression and feasible purification of a thermostable L-2-halo acid dehalogenase of Pseudomonas sp. YL (1995), Biodegradation, 6, 223-227.
    View publication on PubMed

Application

Application Comment Organism
synthesis L-2-haloacid dehalogenase subtype produces D-2-hydroxyalkanoic acids for chiral synthesis in industry in water and organic solvents Pseudomonas sp.

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
27000
-
2 * 27000, SDS-PAGE Pseudomonas sp.
54000
-
SDS-PAGE Pseudomonas sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-chloropropionate + H2O Pseudomonas sp.
-
lactate + HCl
-
?
L-2-haloalkanoic acid + H2O Pseudomonas sp.
-
D-2-hydroxyalkanoic acid + halide
-
?
monobromoacetate + H2O Pseudomonas sp.
-
glycolate + HBr
-
?

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
118
-
-
Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-2-haloacid + H2O
-
Pseudomonas sp. (R)-2-hydroxyacid + halide
-
?
2-chloropropionate + H2O
-
Pseudomonas sp. lactate + HCl
-
?
L-2-haloalkanoic acid + H2O
-
Pseudomonas sp. D-2-hydroxyalkanoic acid + halide
-
?
monobromoacetate + H2O
-
Pseudomonas sp. glycolate + HBr
-
?

Subunits

Subunits Comment Organism
dimer 2 * 27000, SDS-PAGE Pseudomonas sp.