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Literature summary for 3.7.1.3 extracted from

  • Walsh, H.A.; O'Shea, K.C.; Botting, N.P.
    Comparative inhibition by substrate analogues 3-methoxy- and 3-hydroxydesaminokynurenine and an improved 3 step purification of recombinant human kynureninase (2003), BMC Biochem., 4, 13.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.7.1.3

Application

Application Comment Organism
synthesis of inhibitors of kynureninase could prove to be useful in the development of the successful treatment regimen for neurological disorders such as septicemia, AIDS related dementia, Lyme disease, Huntington's and Alzheimer's disease Homo sapiens
synthesis of inhibitors of kynureninase could prove to be useful in the development of the successful treatment regimen for neurological disorders such as septicemia, AIDS related dementia, Lyme disease, Huntington's and Alzheimer's disease Rattus norvegicus
synthesis of inhibitors of kynureninase could prove to be useful in the development of the successful treatment regimen for neurological disorders such as septicemia, AIDS related dementia, Lyme disease, Huntington's and Alzheimer's disease Pseudomonas fluorescens

Cloned(Commentary)

Cloned (Comment) Organism
overexpression is achieved with the Bac-to-Bac baculovirus expression system Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
3,5-dihydroxydeaminokynurenine
-
 Homo sapiens
3-hydroxydeaminokynurenine competitive and non competitive inhibition Homo sapiens
3-hydroxydeaminokynurenine competitive and non competitive inhibition Pseudomonas fluorescens
3-hydroxydeaminokynurenine competitive and non competitive inhibition Rattus norvegicus
3-methoxydeaminokynurenine competitive and non competitive inhibition Homo sapiens
3-methoxydeaminokynurenine competitive and non competitive inhibition Pseudomonas fluorescens
3-methoxydeaminokynurenine competitive and non competitive inhibition Rattus norvegicus
D-kynurenine
-
 Homo sapiens
deaminokynurenine competitive and non competitive inhibition Pseudomonas fluorescens
L-kynurenine
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.003
-
 3-hydroxy-L-kynurenine
-
 Homo sapiens
0.005
-
 3-hydroxy-L-kynurenine
-
 Rattus norvegicus
0.0442
-
 L-kynurenine
-
 Pseudomonas fluorescens
0.2
-
 3-hydroxy-L-kynurenine
-
 Pseudomonas fluorescens
0.5
-
 L-kynurenine
-
 Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52400
-
 recombinant enzyme, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-hydroxy-L-kynurenine + H2O Homo sapiens
-
 3-hydroxyanthranilate + L-alanine
-
 ?
3-hydroxy-L-kynurenine + H2O Rattus norvegicus
-
 3-hydroxyanthranilate + L-alanine
-
 ?
3-hydroxy-L-kynurenine + H2O Pseudomonas fluorescens
-
 3-hydroxyanthranilate + L-alanine
-
 ?
L-kynurenine + H2O Rattus norvegicus L-tryptophan metabolism anthranilate + L-alanine
-
 ?
L-kynurenine + H2O Pseudomonas fluorescens L-tryptophan metabolism anthranilate + L-alanine
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Pseudomonas fluorescens
-
-
-
Rattus norvegicus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no modification
-
 Homo sapiens
no modification
-
 Rattus norvegicus
no modification
-
 Pseudomonas fluorescens

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus
-
 Pseudomonas fluorescens
by strong cationic column, strong anion exchange column and hydroxyapatite column Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.3
-
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxy-L-kynurenine + H2O
-
 Homo sapiens 3-hydroxyanthranilate + L-alanine
-
 ?
3-hydroxy-L-kynurenine + H2O
-
 Rattus norvegicus 3-hydroxyanthranilate + L-alanine
-
 ?
3-hydroxy-L-kynurenine + H2O
-
 Pseudomonas fluorescens 3-hydroxyanthranilate + L-alanine
-
 ?
3-hydroxy-L-kynurenine + H2O the hydroxyl group at the 3-position thus appears to be an important recognition element Homo sapiens 3-hydroxyanthranilate + L-alanine
-
 ?
L-kynurenine + H2O
-
 Rattus norvegicus anthranilate + L-alanine
-
 ?
L-kynurenine + H2O
-
 Pseudomonas fluorescens anthranilate + L-alanine
-
 ?
L-kynurenine + H2O L-tryptophan metabolism Rattus norvegicus anthranilate + L-alanine
-
 ?
L-kynurenine + H2O L-tryptophan metabolism Pseudomonas fluorescens anthranilate + L-alanine
-
 ?

Cofactor

Cofactor Comment Organism Structure
pyridoxal 5'-phosphate
-
 Homo sapiens
pyridoxal 5'-phosphate
-
 Rattus norvegicus
pyridoxal 5'-phosphate
-
 Pseudomonas fluorescens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000005
-
 3-hydroxydeaminokynurenine
-
 Homo sapiens
0.00004
-
 3-hydroxydeaminokynurenine
-
 Rattus norvegicus
0.0001
-
 3-hydroxydeaminokynurenine
-
 Pseudomonas fluorescens
0.00025
-
 3,5-dihydroxydeaminokynurenine
-
 Homo sapiens
0.01
-
 3-methoxydeaminokynurenine
-
 Pseudomonas fluorescens
0.012
-
 D-kynurenine
-
 Homo sapiens
0.012
-
 deaminokynurenine
-
 Pseudomonas fluorescens
0.015
-
 3-methoxydeaminokynurenine
-
 Homo sapiens
0.02
-
 L-kynurenine
-
 Homo sapiens
0.175
-
 2-amino-4-(3-methoxyphenyl)-4-oxobutanoic acid
-
 Rattus norvegicus