Any feedback?
Literature summary for 3.6.5.1 extracted from
- Studies on the polypeptide elongation factor 2 from Sulfolobus solfataricus. Interaction with guanosine nucleotides and GTPase activity stimulated by ribosomes (1995), J. Biol. Chem., 270, 21082-21085.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | GTPase activity is stimulated by ribosomes up to 2000fold | Saccharolobus solfataricus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| GDP | competitive inhibition | Saccharolobus solfataricus |  |
| Gpp(NH)p | competitive inhibition | Saccharolobus solfataricus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.008 | - |
GTP | 50°C | Saccharolobus solfataricus | |
| 0.009 | - |
GTP | 60°C | Saccharolobus solfataricus | |
| 0.01 | - |
GTP | 70°C | Saccharolobus solfataricus | |
| 0.011 | - |
GTP | 75°C | Saccharolobus solfataricus | |
| 0.013 | - |
GTP | 80°C | Saccharolobus solfataricus | |
| 0.032 | - |
GTP | 87°C | Saccharolobus solfataricus | |
| 0.148 | - |
GTP | 91°C | Saccharolobus solfataricus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| ribosome | - |
Saccharolobus solfataricus | 5840 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + H2O | Saccharolobus solfataricus | - |
GDP + phosphate | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purified from Sulfolobus solfataricus | Saccharolobus solfataricus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, Tris-HCl buffer, pH 7.8, 10 mM MgCl2, 50% v/v glycerol | Saccharolobus solfataricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GTP + H2O | - |
Saccharolobus solfataricus | GDP + phosphate | - |
ir | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| elongation factor 2 | - |
Saccharolobus solfataricus |
| heterotrimeric G-protein GTPase | - |
Saccharolobus solfataricus |
| SsEF-2 | - |
Saccharolobus solfataricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
- |
Saccharolobus solfataricus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
GTP | 50°C | Saccharolobus solfataricus | |
| 0.8 | - |
GTP | 60°C | Saccharolobus solfataricus | |
| 1.7 | - |
GTP | 70°C | Saccharolobus solfataricus | |
| 2.7 | - |
GTP | 75°C | Saccharolobus solfataricus | |
| 3.5 | - |
GTP | 80°C | Saccharolobus solfataricus | |
| 6.2 | - |
GTP | 87°C | Saccharolobus solfataricus | |
| 8.8 | - |
GTP | 91°C | Saccharolobus solfataricus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
- |
Saccharolobus solfataricus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
