Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Upf2 | interaction analysis. The two proteins Upf1 and Upf2 form a complex while unwinding RNA. The binding of Upf2 to CH domain activates Upf1-CH-HD unwinding and translocation | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
recombinant expression of C-terminally His-tagged and N-terminally calmodulin-binding-protein-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Rosetta | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
F192E | site-directed mutagenesis, the mutant shows slightly reduced activity compared with the wild-type enzyme. Upf1F192E definitely prefers to unwind a dsDNA than to translocate it, strand switching | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | Homo sapiens | - |
ADP + phosphate | - |
? | |
additional information | Homo sapiens | human Upf1 is able to translocate slowly over long single-stranded nucleic acids with a high processivity. Upf1 efficiently translocates through double-stranded structures and protein-bound sequences. The helicase domain of Upf1 is capable of both unwinding double-stranded nucleic acids and translocation on single-stranded nucleic acids over long distances. Upf1 remodels nucleoprotein complexes | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q92900 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant C-terminally His-tagged and N-terminally calmodulin-binding-protein-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) Rosetta by nickel and calmodulin affinity chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O | - |
Homo sapiens | ADP + phosphate | - |
? | |
additional information | human Upf1 is able to translocate slowly over long single-stranded nucleic acids with a high processivity. Upf1 efficiently translocates through double-stranded structures and protein-bound sequences. The helicase domain of Upf1 is capable of both unwinding double-stranded nucleic acids and translocation on single-stranded nucleic acids over long distances. Upf1 remodels nucleoprotein complexes | Homo sapiens | ? | - |
? | |
additional information | Upf1-HD is a slow translocase compared with other monomeric helicases like UvrD and NS3, but is able to translocate slowly over long single-stranded nucleic acids with a high processivity which exceeds the size of the tested hairpins, overview. Enzyme Upf1 is able to both unwind a long dsRNA of 156 bp and translocate onto ssRNA. Active translocating enzyme Upf1 disrupts protein-nucleic acid interactions. Upf1 remodels nucleoprotein complexes. Substrates are RNADNA hybrids, ssDNA and ssRNA | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Upf1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
26 | - |
RNA assay at | Homo sapiens |
37 | - |
DNA assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | modeling of the late phases of nonsense-mediated mRNA decay | Homo sapiens |
physiological function | Upf1 is a RNA helicase essential for nonsense-mediated mRNA decay, Upf1 is an efficient ribonucleoprotein complex remodeler. Upf1, once recruited onto nonsense-mediated mRNA decay targets, can scan the entire transcript to irreversibly remodel the mRNP, facilitating its degradation by the nonsense-mediated mRNA decay machinery. The ATPase activity of Upf1 is required for the correct progression of nonsense-mediated mRNA decay, a quality-control mechanism that eliminates mRNAs containing premature translation termination codons and also regulates the expression of numerous mRNAs carrying nonsense-mediated mRNA decay target features. The enzyme is specifically recruited to specific targets by an intricate stepwise and translation-dependent pathway | Homo sapiens |