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Literature summary for 3.6.4.10 extracted from

  • Slepenkov, S.; Witt, S.N.
    Kinetics of the reactions of the Escherichia coli molecular chaperone DnaK with ATP: Evidence that a three-step reaction precedes ATP hydrolysis (1998), Biochemistry, 37, 1015-1024.
    View publication on PubMed

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
1 * 44000 + 1 * 23000, 44-kDa ATP binding domain and 23-kDA COOH-terminal domain Escherichia coli
44000
-
1 * 44000 + 1 * 23000, 44-kDa ATP binding domain and 23-kDA COOH-terminal domain Escherichia coli
70000
-
-
Zea mays
70000
-
-
Solanum lycopersicum
70000
-
DnaK Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O Escherichia coli
-
ADP + phosphate
-
?
ATP + H2O Zea mays
-
ADP + phosphate
-
?
ATP + H2O Solanum lycopersicum
-
ADP + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Solanum lycopersicum
-
tomato
-
Zea mays
-
maize
-

Reaction

Reaction Comment Organism Reaction ID
ATP + H2O = ADP + phosphate highly diverse group of enzymes that perform many functions that are similar to those of chaperonins. They comprise a number of heat-shock-cognate proteins. They are also active in clathrin uncoating and in the oligomerization of actin Escherichia coli
ATP + H2O = ADP + phosphate highly diverse group of enzymes that perform many functions that are similar to those of chaperonins. They comprise a number of heat-shock-cognate proteins. They are also active in clathrin uncoating and in the oligomerization of actin Zea mays
ATP + H2O = ADP + phosphate highly diverse group of enzymes that perform many functions that are similar to those of chaperonins. They comprise a number of heat-shock-cognate proteins. They are also active in clathrin uncoating and in the oligomerization of actin Solanum lycopersicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O
-
Escherichia coli ADP + phosphate
-
?
ATP + H2O
-
Zea mays ADP + phosphate
-
?
ATP + H2O
-
Solanum lycopersicum ADP + phosphate
-
?

Subunits

Subunits Comment Organism
dimer 1 * 44000 + 1 * 23000, 44-kDa ATP binding domain and 23-kDA COOH-terminal domain Escherichia coli

Synonyms

Synonyms Comment Organism
BiP
-
Solanum lycopersicum
DnaK
-
Escherichia coli

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 37
-
Escherichia coli