Cloned (Comment) | Organism |
---|---|
- |
Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
hanging-drop vapor-diffusion method at 20°C. Structures of DcpS in ligand-free form and in a complex with m7GDP. Examination of the crystallographic B-factors indicates that the N-terminal domain in apo-DcpS is inherently flexible, and in a dynamic state ready for substrate binding and product release | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
m7G5'ppp5'N(3'ppp5'N)n + H2O | Homo sapiens | eukaryotic cells utilize DcpS, a scavenger decapping enzyme, to degrade the residual cap structure following 30-50 mRNA decay, thereby preventing the premature decapping of the capped long mRNA and misincorporation of methylated nucleotides in nucleic acids | 7-methylguanosine 5'-phosphate + pp5'N(3'ppp5'N)n | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q96C86 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a 5'-(N7-methyl 5'-triphosphoguanosine)-[mRNA] + H2O = N7-methylguanosine 5'-phosphate + a 5'-diphospho-[mRNA] | (1) | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7-methylguanosine 5'-diphosphate + H2O | - |
Homo sapiens | 7-methylguanosine 5'-phosphate + phosphate | - |
? | |
m7G5'ppp5'N(3'ppp5'N)n + H2O | eukaryotic cells utilize DcpS, a scavenger decapping enzyme, to degrade the residual cap structure following 30-50 mRNA decay, thereby preventing the premature decapping of the capped long mRNA and misincorporation of methylated nucleotides in nucleic acids | Homo sapiens | 7-methylguanosine 5'-phosphate + pp5'N(3'ppp5'N)n | - |
? | |
m7G5'ppp5'N(3'ppp5'N)n + H2O | Tyr273 seems to play an important role in cap binding and product release | Homo sapiens | 7-methylguanosine 5'-phosphate + pp5'N(3'ppp5'N)n | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | in contrast, and similar to the m7GpppG-DcpS complex, DcpS with bound m7GDP is an asymmetric dimer in which the closed state appears to be the substrate-bound complex, whereas the open state mimics the product-bound complex. apo-DcpS is a symmetric dimer | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
Dcps | - |
Homo sapiens |
m7GpppN m7GMP phosphohydrolase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | eukaryotic cells utilize DcpS, a scavenger decapping enzyme, to degrade the residual cap structure following 30-50 mRNA decay, thereby preventing the premature decapping of the capped long mRNA and misincorporation of methylated nucleotides in nucleic acids | Homo sapiens |