Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Dcs2 protein | Dcs2 is recruited into cytoplasmic P bodies, its inhibitory function may be focused in these centres of mRNA storage/turnover. Dcs2 is a stress-induced regulatory protein that modulates m7GpppX pyrophosphatase activity | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00014 | - |
m7G5'ppp5'G | pH 7.0, 30°C, Dcs1 homodimer | Saccharomyces cerevisiae | |
0.00026 | - |
7-methylguanosine 5'-diphosphate | pH 7.0, 30°C, Dcs1 homodimer | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
7-methylguanosine 5'-diphosphate + H2O | Saccharomyces cerevisiae | cleavage of 7-methylguanosine 5'-diphosphate generated by Dcp1/Dcp2-mediated decapping in the 5' to 3' decay pathway | 7-methylguanosine 5'-phosphate + phosphate | - |
? | |
m7G5'ppp5'N(3'ppp5'N)n + H2O | Saccharomyces cerevisiae | cleavage of 5' end m7G-oligoribonucleotide fragments generated by 3' to 5' exonucleolytic decay | 7-methylguanosine 5'-phosphate + pp5'N(3'ppp5'N)n | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | Q06151 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7-methylguanosine 5'-diphosphate + H2O | - |
Saccharomyces cerevisiae | 7-methylguanosine 5'-phosphate + phosphate | - |
? | |
7-methylguanosine 5'-diphosphate + H2O | cleavage of 7-methylguanosine 5'-diphosphate generated by Dcp1/Dcp2-mediated decapping in the 5' to 3' decay pathway | Saccharomyces cerevisiae | 7-methylguanosine 5'-phosphate + phosphate | - |
? | |
m7G5'ppp5'G + H2O | - |
Saccharomyces cerevisiae | 7-methylguanosine 5'-phosphate + ? | - |
? | |
m7G5'ppp5'N(3'ppp5'N)n + H2O | cleavage of 5' end m7G-oligoribonucleotide fragments generated by 3' to 5' exonucleolytic decay | Saccharomyces cerevisiae | 7-methylguanosine 5'-phosphate + pp5'N(3'ppp5'N)n | - |
? | |
m7G5'ppp5'N(3'ppp5'N)n + H2O | n = 1-8 | Saccharomyces cerevisiae | 7-methylguanosine 5'-phosphate + pp5'N(3'ppp5'N)n | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | Dcs1 is active as a homodimer. Dcs2 protein forms a heterodimer together with Dcs1, both modulating Dcs1 substrate specificity and suppressing its turnover number | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
Dcps | - |
Saccharomyces cerevisiae |
Dcs1 | - |
Saccharomyces cerevisiae |
m7GpppX pyrophosphatase | - |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0017 | - |
7-methylguanosine 5'-diphosphate | pH 7.0, 30°C, Dcs1 homodimer | Saccharomyces cerevisiae | |
0.012 | - |
m7G5'ppp5'G | pH 7.0, 30°C, Dcs1 homodimer | Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
malfunction | depletion of Dcs1, reduces chronological life span, possibly by modulating m7G misincorporation into nucleic acids. This can potentially link control of mRNA metabolism with senescence | Saccharomyces cerevisiae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6.5 | - |
7-methylguanosine 5'-diphosphate | pH 7.0, 30°C, Dcs1 homodimer | Saccharomyces cerevisiae | |
86 | - |
m7G5'ppp5'G | pH 7.0, 30°C, Dcs1 homodimer | Saccharomyces cerevisiae |