Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | inhibits the cleavage of diphosphate in the presence of 2.5 mM Mg2+. 0.01 mM CaCl2 inhibits the enzyme by about 50% | Thermoplasma acidophilum | |
Phenylglyoxal | the inhibitory effect of phenylglyoxal is higher at 56°C than at room temperature. With 5 mM phenylglyoxal almost complete inhibition can be observed after 15 min incubation at 56°C | Thermoplasma acidophilum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
diphosphate | pH 6.2, 56°C | Thermoplasma acidophilum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | the enzyme occurs almost exclusively in the cytoplasmic fraction | Thermoplasma acidophilum | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | absolute requirement for divalent cations, 2.5 mM Mn2+ activates 37% compared to 2.5 mM Mg2+ | Thermoplasma acidophilum | |
Mg2+ | absolute requirement for divalent cations for catalytic action, Mg2 + (2.5 mM) conferring the highest activity. KM for Mg2+ is approximately 1.7 mM | Thermoplasma acidophilum | |
Mn2+ | absolute requirement for divalent cations, 2.5 mM Mn2+ activates 17% compared to 2.5 mM Mg2+ | Thermoplasma acidophilum | |
Zn2+ | absolute requirement for divalent cations, 2.5 mM Mn2+ activates 21% compared to 2.5 mM Mg2+ | Thermoplasma acidophilum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22000 | - |
6 * 22000, SDS-PAGE | Thermoplasma acidophilum |
110000 | - |
gel filtration | Thermoplasma acidophilum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoplasma acidophilum | P37981 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermoplasma acidophilum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1200 | - |
pH 6.2, 56°C | Thermoplasma acidophilum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + H2O | pronounced specificity for diphosphate | Thermoplasma acidophilum | 2 phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | 6 * 22000, SDS-PAGE | Thermoplasma acidophilum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
- |
Thermoplasma acidophilum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 100 | 70°C: about 70% of maximal activity, 100°C: about 80% of maximal activity | Thermoplasma acidophilum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2200 | - |
diphosphate | pH 6.2, 56°C | Thermoplasma acidophilum |