Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta(DE3) cells | Sulfurisphaera tokodaii |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 16% (w/v) PEG 10000, 0.1 M bis-Tris pH 5.3 and 0.1 M ammonium acetate | Sulfurisphaera tokodaii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-iminobutanoate + H2O | Sulfurisphaera tokodaii | - |
2-oxobutanoate + NH3 | - |
? | |
2-iminobutanoate + H2O | Sulfurisphaera tokodaii 7 | - |
2-oxobutanoate + NH3 | - |
? | |
2-iminopropanoate + H2O | Sulfurisphaera tokodaii | - |
pyruvate + NH3 | - |
? | |
2-iminopropanoate + H2O | Sulfurisphaera tokodaii 7 | - |
pyruvate + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfurisphaera tokodaii | Q973T6 | - |
- |
Sulfurisphaera tokodaii 7 | Q973T6 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography, and Superdex 75 gel filtration | Sulfurisphaera tokodaii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-iminobutanoate + H2O | - |
Sulfurisphaera tokodaii | 2-oxobutanoate + NH3 | - |
? | |
2-iminobutanoate + H2O | - |
Sulfurisphaera tokodaii 7 | 2-oxobutanoate + NH3 | - |
? | |
2-iminopropanoate + H2O | - |
Sulfurisphaera tokodaii | pyruvate + NH3 | - |
? | |
2-iminopropanoate + H2O | - |
Sulfurisphaera tokodaii 7 | pyruvate + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ST0811 | - |
Sulfurisphaera tokodaii |