Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-AI(DE3) cells | Thiobacillus thioparus |
Crystallization (Comment) | Organism |
---|---|
mutant R136W, hanging drop vapor diffusion method, using 0.1 M potassium phosphate (pH 7.2) and 1.4 M sodium potassium tartrate | Thiobacillus thioparus |
Protein Variants | Comment | Organism |
---|---|---|
R136W | the mutant of the gamma subunit shows no thiocyanate hydrolysis activity but does catalyze the hydration of nitriles | Thiobacillus thioparus |
R90F | the mutant of the beta subunit shows no thiocyanate hydrolysis activity but does catalyze the hydration of nitriles | Thiobacillus thioparus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
11 | - |
Methacrylonitrile | wild type enzyme, at pH 7.5 and 27°C | Thiobacillus thioparus | |
93 | - |
Methacrylonitrile | mutant enzyme R90F, at pH 7.5 and 27°C | Thiobacillus thioparus | |
160 | - |
Methacrylonitrile | mutant enzyme R136W, at pH 7.5 and 27°C | Thiobacillus thioparus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co3+ | contains cobalt | Thiobacillus thioparus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
thiocyanate + H2O | Thiobacillus thioparus | - |
carbonyl sulfide + NH3 + HO- | - |
? | |
thiocyanate + H2O | Thiobacillus thioparus THI115 | - |
carbonyl sulfide + NH3 + HO- | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thiobacillus thioparus | O66187 and O66186 and O66188 | O66187 subunit alpha, O66186 subunit beta, O66188 subunit gamma | - |
Thiobacillus thioparus THI115 | O66187 and O66186 and O66188 | O66187 subunit alpha, O66186 subunit beta, O66188 subunit gamma | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methacrylonitrile + H2O | - |
Thiobacillus thioparus | ? | - |
? | |
methacrylonitrile + H2O | - |
Thiobacillus thioparus THI115 | ? | - |
? | |
thiocyanate + H2O | - |
Thiobacillus thioparus | carbonyl sulfide + NH3 + HO- | - |
? | |
thiocyanate + H2O | - |
Thiobacillus thioparus THI115 | carbonyl sulfide + NH3 + HO- | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SCNase | - |
Thiobacillus thioparus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
Methacrylonitrile | mutant enzyme R136W, at pH 7.5 and 27°C | Thiobacillus thioparus | |
2.1 | - |
Methacrylonitrile | mutant enzyme R90F, at pH 7.5 and 27°C | Thiobacillus thioparus | |
57 | - |
Methacrylonitrile | wild type enzyme, at pH 7.5 and 27°C | Thiobacillus thioparus |