Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Thiobacillus thioparus |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 0.1 M potassium phosphate (pH 7.2) containing 2.0 M ammonium sulfate for purified SCNase or 0.1 M potassium phosphate (pH 6.9) containing 1.5 M Na/K tartrate for spontaneously activated SCNase and inactivated SCNase, respectively | Thiobacillus thioparus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thiobacillus thioparus | O66186 | subunit beta | - |
Thiobacillus thioparus | O66187 | subunit alpha | - |
Thiobacillus thioparus | O66188 | subunit gamma | - |
Oxidation Stability | Organism |
---|---|
when recombinant SCNase, containing gammaCys131-SO2 and gammaCys133-SO(H) modifications in the cobalt center, is stored at 20°C under aerobic conditions for 4 months, no enzymatic activity is detected | Thiobacillus thioparus |
when recombinant SCNase, containing gammaCys131-SO2- and gammaCys133-SO(H) modifications in the cobalt center, is stored at 20°C under aerobic conditions for 4 months, no enzymatic activity is detected | Thiobacillus thioparus |
Purification (Comment) | Organism |
---|---|
- |
Thiobacillus thioparus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
thiocyanate + H2O | - |
Thiobacillus thioparus | carbonyl sulfide + NH3 + OH- | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SCNase | - |
Thiobacillus thioparus |