Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.5.5.8 extracted from

  • Arakawa, T.; Kawano, Y.; Kataoka, S.; Katayama, Y.; Kamiya, N.; Yohda, M.; Odaka, M.
    Structure of thiocyanate hydrolase: a new nitrile hydratase family protein with a novel five-coordinate cobalt(III) center (2007), J. Mol. Biol., 366, 1497-1509.
    View publication on PubMed
Search for more literature for 3.5.5.8

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method with 0.1 M potassium phosphate (pH 7.6) containing 1.5 M Na/K L-(+) tartrate or 0.1 M potassium phosphate (pH 7.2) containing 2 M ammonium sulfate for apo- or native SCNase, respectively Thiobacillus thioparus

Metals/Ions

Metals/Ions Comment Organism Structure
Co3+ contains a five-coordinate cobalt(III) center Thiobacillus thioparus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
240000
-
 gel filtration Thiobacillus thioparus

Organism

Organism UniProt Comment Textmining
Thiobacillus thioparus
-
-
-
Thiobacillus thioparus THI115
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thiobacillus thioparus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
thiocyanate + H2O
-
 Thiobacillus thioparus carbonyl sulfide + NH3 + OH-
-
 ?
thiocyanate + H2O
-
 Thiobacillus thioparus THI115 carbonyl sulfide + NH3 + OH-
-
 ?

Subunits

Subunits Comment Organism
heterododecamer x-ray crystallography Thiobacillus thioparus

Synonyms

Synonyms Comment Organism
SCNase
-
 Thiobacillus thioparus