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Literature summary for 3.5.4.B9 extracted from

  • Lu, X.; Zhang, T.; Xu, Z.; Liu, S.; Zhao, B.; Lan, W.; Wang, C.; Ding, J.; Cao, C.
    Crystal structure of DNA cytidine deaminase ABOBEC3G catalytic deamination domain suggests a binding mode of full-length enzyme to single-stranded DNA (2015), J. Biol. Chem., 290, 4010-4021.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.4.B9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta(DE3)plys and BW310 cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
head-to-tail dimer of the A3G catalytic deamination domain A3G-CD2, sitting drop vapor diffusion method, using 0.1 M sodium citrate tribasic dehydrate, pH 5.6, 20% (v/v) 2-propanol, 20% (w/v) polyethylene glycol 4000 Homo sapiens

Protein Variants

Protein Variants Comment Organism
D264A the variant has 5% of the catalytic efficiency of the wild type protein Homo sapiens
D370A the variant has 16% of the catalytic efficiency of the wild type protein Homo sapiens
F268A the variant has 25% of the catalytic efficiency of the wild type protein Homo sapiens
H248G the variant has 158% of the catalytic efficiency of the wild type protein Homo sapiens
H250A the variant has 266% of the catalytic efficiency of the wild type protein Homo sapiens
P210A the variant has 1% of the catalytic efficiency of the wild type protein Homo sapiens
P210G the variant has 10% of the catalytic efficiency of the wild type protein Homo sapiens
Q245A the mutation nearly abolishes the catalytic efficiency to 5% compared to the wild type protein Homo sapiens
Q380A the variant has 68% of the catalytic efficiency of the wild type protein Homo sapiens
R256A the mutation nearly abolishes the catalytic efficiency to 1% compared to the wild type protein Homo sapiens
R374A the variant has 3% of the catalytic efficiency of the wild type protein Homo sapiens
R376A the variant has 15% of the catalytic efficiency of the wild type protein Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01615
-
 cytosine in single-stranded viral DNA mutant enzyme Q380A, at pH 7.5 and 37°C Homo sapiens
0.01846
-
 cytosine in single-stranded viral DNA mutant enzyme P210G, at pH 7.5 and 37°C Homo sapiens
0.02042
-
 cytosine in single-stranded viral DNA wild type enzyme, at pH 7.5 and 37°C Homo sapiens
0.02252
-
 cytosine in single-stranded viral DNA mutant enzyme F268A, at pH 7.5 and 37°C Homo sapiens
0.02705
-
 cytosine in single-stranded viral DNA mutant enzyme R374A, at pH 7.5 and 37°C Homo sapiens
0.03306
-
 cytosine in single-stranded viral DNA mutant enzyme H248G, at pH 7.5 and 37°C Homo sapiens
0.03325
-
 cytosine in single-stranded viral DNA mutant enzyme H250A, at pH 7.5 and 37°C Homo sapiens
0.06323
-
 cytosine in single-stranded viral DNA mutant enzyme R376A, at pH 7.5 and 37°C Homo sapiens
0.08188
-
 cytosine in single-stranded viral DNA mutant enzyme P210A, at pH 7.5 and 37°C Homo sapiens
0.1189
-
 cytosine in single-stranded viral DNA mutant enzyme D370A, at pH 7.5 and 37°C Homo sapiens
0.1639
-
 cytosine in single-stranded viral DNA mutant enzyme R256A, at pH 7.5 and 37°C Homo sapiens
0.2785
-
 cytosine in single-stranded viral DNA mutant enzyme Q245A, at pH 7.5 and 37°C Homo sapiens
0.3944
-
 cytosine in single-stranded viral DNA mutant enzyme D264A, at pH 7.5 and 37°C Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cytosine in single-stranded viral DNA + H2O Homo sapiens
-
 uracil in single-stranded viral DNA + NH3
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9HC16
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex75 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cytosine in single-stranded viral DNA + H2O
-
 Homo sapiens uracil in single-stranded viral DNA + NH3
-
 ?

Synonyms

Synonyms Comment Organism
A3G
-
 Homo sapiens
APOBEC3G
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.000083
-
 cytosine in single-stranded viral DNA mutant enzyme R374A, at pH 7.5 and 37°C Homo sapiens
0.000102
-
 cytosine in single-stranded viral DNA mutant enzyme P210A, at pH 7.5 and 37°C Homo sapiens
0.000165
-
 cytosine in single-stranded viral DNA mutant enzyme P210G, at pH 7.5 and 37°C Homo sapiens
0.00017
-
 cytosine in single-stranded viral DNA mutant enzyme D370A, at pH 7.5 and 37°C Homo sapiens
0.0002
-
 cytosine in single-stranded viral DNA mutant enzyme R256A, at pH 7.5 and 37°C Homo sapiens
0.000517
-
 cytosine in single-stranded viral DNA mutant enzyme F268A, at pH 7.5 and 37°C Homo sapiens
0.00085
-
 cytosine in single-stranded viral DNA mutant enzyme R376A, at pH 7.5 and 37°C Homo sapiens
0.001
-
 cytosine in single-stranded viral DNA mutant enzyme Q380A, at pH 7.5 and 37°C Homo sapiens
0.0014
-
 cytosine in single-stranded viral DNA mutant enzyme Q245A, at pH 7.5 and 37°C Homo sapiens
0.0018
-
 cytosine in single-stranded viral DNA wild type enzyme, at pH 7.5 and 37°C Homo sapiens
0.002
-
 cytosine in single-stranded viral DNA mutant enzyme D264A, at pH 7.5 and 37°C Homo sapiens
0.0047
-
 cytosine in single-stranded viral DNA mutant enzyme H248G, at pH 7.5 and 37°C Homo sapiens
0.008
-
 cytosine in single-stranded viral DNA mutant enzyme H250A, at pH 7.5 and 37°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0012
-
 cytosine in single-stranded viral DNA mutant enzyme P210A, at pH 7.5 and 37°C Homo sapiens
0.0012
-
 cytosine in single-stranded viral DNA mutant enzyme R256A, at pH 7.5 and 37°C Homo sapiens
0.0031
-
 cytosine in single-stranded viral DNA mutant enzyme R374A, at pH 7.5 and 37°C Homo sapiens
0.0046
-
 cytosine in single-stranded viral DNA mutant enzyme D264A, at pH 7.5 and 37°C Homo sapiens
0.005
-
 cytosine in single-stranded viral DNA mutant enzyme Q245A, at pH 7.5 and 37°C Homo sapiens
0.009
-
 cytosine in single-stranded viral DNA mutant enzyme P210G, at pH 7.5 and 37°C Homo sapiens
0.0135
-
 cytosine in single-stranded viral DNA mutant enzyme R376A, at pH 7.5 and 37°C Homo sapiens
0.01425
-
 cytosine in single-stranded viral DNA mutant enzyme H248G, at pH 7.5 and 37°C Homo sapiens
0.0143
-
 cytosine in single-stranded viral DNA mutant enzyme D370A, at pH 7.5 and 37°C Homo sapiens
0.023
-
 cytosine in single-stranded viral DNA mutant enzyme F268A, at pH 7.5 and 37°C Homo sapiens
0.062
-
 cytosine in single-stranded viral DNA mutant enzyme Q380A, at pH 7.5 and 37°C Homo sapiens
0.09
-
 cytosine in single-stranded viral DNA wild type enzyme, at pH 7.5 and 37°C Homo sapiens
0.24
-
 cytosine in single-stranded viral DNA mutant enzyme H250A, at pH 7.5 and 37°C Homo sapiens