Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells and in Sf9 insect cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
carboxy-terminal deaminase domain 2 of APOBEC3G (residues 197-380), hanging drop vapor diffusion method, using 100 mM MES, pH 6.5, 40% (w/v) PEG 200, at 18°C | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D316R/D317R | the mutant shows about 180% deamination activity and about 200% single-stranded DNA binding compared to the wild type enzyme | Homo sapiens |
F298A | the mutant shows about 10% deamination activity compared to the wild type enzyme | Homo sapiens |
N244A | the mutant shows no deamination activity | Homo sapiens |
R213E | the mutant shows about 3% deamination activity compared to the wild type enzyme | Homo sapiens |
R215E | the mutant shows no deamination activity | Homo sapiens |
R256E | the mutant shows about 3% deamination activity compared to the wild type enzyme | Homo sapiens |
R313E/R320D | the mutant shows no deamination activity and about 75% single-stranded DNA binding compared to the wild type enzyme | Homo sapiens |
R374E/R376D | the mutant shows less than 10% deamination activity and about 50% single-stranded DNA binding compared to the wild type enzyme | Homo sapiens |
W285A | the mutant shows no deamination activity | Homo sapiens |
Y315A | the mutant shows no deamination activity | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | contains a zinc atom in the active site | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
cytosine in single-stranded viral DNA + H2O | Homo sapiens | the full-length enzyme processively deaminates cytidine in two 5'-CCC-3' motifs located on a single-stranded DNA substrate, during one binding event. The full-length enzyme also exerts a 3' to 5' deamination bias by preferentially deaminating the cytidine in the CCC motif near the 5'end of the single-stranded DNA substrate | uracil in single-stranded viral DNA + NH3 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9HC16 | - |
- |
Purification (Comment) | Organism |
---|---|
Superdex 75 gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cytosine in single-stranded viral DNA + H2O | the full-length enzyme processively deaminates cytidine in two 5'-CCC-3' motifs located on a single-stranded DNA substrate, during one binding event. The full-length enzyme also exerts a 3' to 5' deamination bias by preferentially deaminating the cytidine in the CCC motif near the 5'end of the single-stranded DNA substrate | Homo sapiens | uracil in single-stranded viral DNA + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
APOBEC3G | - |
Homo sapiens |