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Literature summary for 3.5.4.B9 extracted from

  • Furukawa, A.; Nagata, T.; Matsugami, A.; Habu, Y.; Sugiyama, R.; Hayashi, F.; Kobayashi, N.; Yokoyama, S.; Takaku, H.; Katahira, M.
    Structure, interaction and real-time monitoring of the enzymatic reaction of wild-type APOBEC3G (2009), EMBO J., 28, 440-451.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3/RIL) cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
F202A the mutation causes a decrease of the enzyme activity Homo sapiens
F241K the mutation causes a decrease of the enzyme activity Homo sapiens
L235A the mutation causes a decrease of the enzyme activity Homo sapiens
L235K the mutation causes a decrease of the enzyme activity Homo sapiens
L242A the mutation causes a decrease of the enzyme activity Homo sapiens
L242K the mutation causes a decrease of the enzyme activity Homo sapiens
T203A the mutation causes a decrease of the enzyme activity Homo sapiens
V233A the mutation causes a decrease of the enzyme activity Homo sapiens
V233K the mutation causes a decrease of the enzyme activity Homo sapiens
W232A the mutation causes a decrease of the enzyme activity Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens Q9HC16
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-

Purification (Commentary)

Purification (Comment) Organism
Ni Sepharose resin column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cytosine in single-stranded viral DNA + H2O minus strand deamination occurs preferentially at a CCCA sequence. The third cytidine of the d(CCCA) segment is deaminated at an early stage and that then the second one is deaminated at a late stage, so the deamination is carried out in a strict 3'-5' order Homo sapiens uracil in single-stranded viral DNA + NH3
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Synonyms

Synonyms Comment Organism
APOBEC3G
-
Homo sapiens

General Information

General Information Comment Organism
physiological function the enzyme exhibits anti-human immunodeficiency virus-1 (HIV-1) activity by deaminating cytidines of the minus strand of HIV-1. Virus infectivity factor of HIV-1 counteracts the anti-HIV-1 activity of the enzyme Homo sapiens