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Literature summary for 3.5.4.16 extracted from

  • Sankaran, B.; Bonnett, S.A.; Shah, K.; Gabriel, S.; Reddy, R.; Schimmel, P.; Rodionov, D.A.; de Crecy-Lagard, V.; Helmann, J.D.; Iwata-Reuyl, D.; Swairjo, M.A.
    Zinc-independent folate biosynthesis: genetic, biochemical, and structural investigations reveal new metal dependence for GTP cyclohydrolase IB (2009), J. Bacteriol., 191, 6936-6949.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.4.16

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli B834(DE3) cells Bacillus subtilis

Crystallization (Commentary)

Crystallization (Comment) Organism
selenomethionine-labeled isozyme GCYH-IB, sitting drop vapor diffusion method, using polyethylene glycol 6000 (10-16% (w/v)), LiCl (1-1.4 M), Tris (50 mM, pH 9.0), and Tris-HCl (50 mM, pH 7.0) Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ no activity detected regardless of metal concentration Bacillus subtilis
Cd2+ no activity detected regardless of metal concentration Bacillus subtilis
Co2+ 24% residual activity at 0.1 mM Bacillus subtilis
Co3+ no activity detected regardless of metal concentration Bacillus subtilis
Cu2+ no activity detected regardless of metal concentration Bacillus subtilis
EDTA no activity of GCYH-IB is observed in the presence of EDTA Bacillus subtilis
Fe2+ 75% residual activity at 1 mM Bacillus subtilis
Fe3+ no activity detected regardless of metal concentration Bacillus subtilis
Mg2+ 43% residual activity at 0.1 mM Bacillus subtilis
Ni2+ 9.8% residual activity at 0.1 mM Bacillus subtilis
Zn2+ 14% residual activity at 0.05 mM Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00987
-
 GTP isozyme GCYH-IB, in 100 mM HEPES (pH 8.0), 100 mM KCl, 0.5 mM MnCl2, 1 mM dithiothreitol, temperature not specified in the publication Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ required for activity of isozyme GCYH-1B Bacillus subtilis
Mn2+ required for activity of isozyme GCYH-1B Neisseria gonorrhoeae
Zn2+ isozyme GCYH-1A is a Zn2+-dependent enzyme, isozyme GCYH-IB is expressed only under Zn2+-limiting conditions Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28568
-
 4 * 28568, isozyme GCYH-1B, apparent molecular weight estimated from gel filtration Neisseria gonorrhoeae
108600
-
 isozyme GCYH-1B, apparent molecular weight estimated from gel filtration Neisseria gonorrhoeae

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Neisseria gonorrhoeae Q5F9K6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O
-
 Bacillus subtilis formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate
-
 ?
GTP + H2O
-
 Neisseria gonorrhoeae formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate
-
 ?

Subunits

Subunits Comment Organism
homodecamer isozyme GCYH-1A Bacillus subtilis
homotetramer 4 * 28568, isozyme GCYH-1B, apparent molecular weight estimated from gel filtration Neisseria gonorrhoeae
homotetramer isozyme GCYH-1B Bacillus subtilis

Synonyms

Synonyms Comment Organism
GCYH-IA isozyme Bacillus subtilis
GCYH-IB isozyme Bacillus subtilis
GCYH-IB isozyme Neisseria gonorrhoeae
GTP cyclohydrolase IA isozyme Bacillus subtilis
GTP cyclohydrolase IB isozyme Bacillus subtilis
GTP cyclohydrolase IB isozyme Neisseria gonorrhoeae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0011
-
 GTP isozyme GCYH-IB, in 100 mM HEPES (pH 8.0), 100 mM KCl, 0.5 mM MnCl2, 1 mM dithiothreitol, temperature not specified in the publication Bacillus subtilis

General Information

General Information Comment Organism
physiological function isozyme GCYH-IB functions to allow folate biosynthesis during Zn2+ starvation Neisseria gonorrhoeae
physiological function isozyme GCYH-IB functions to allow folate biosynthesis during Zn2+ starvation, isozyme GCYH-IB functionally replaces GCYH-IA in Bacillus subtilis under zinc-limiting conditions Bacillus subtilis