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Literature summary for 3.5.4.16 extracted from

  • Auerbach, G.; Herrmann, A.; Bracher, A.; Bader, G.; Gutlich, M.; Fischer, M.; Neukamm, M.; Garrido-Franco, M.; Richardson, J.; Nar, H.; Huber, R.; Bacher, A.
    Zinc plays a key role in human and bacterial GTP cyclohydrolase I (2000), Proc. Natl. Acad. Sci. USA, 97, 13567-13572.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.5.4.16

Cloned(Commentary)

Cloned (Comment) Organism
expression of DELTA42-hGTP-CH-I in Escherichia coli M15 Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
4.6 mg/ml purified recombinant DELTA42-hGTP-CH-I in 0.1 M potassium phosphate, pH 7.0, 0.02% NaN3, mixed with precipitation solution containing 6% PEG 6000, 150 mM KCl, 100 mM MOPS, pH 7.0, equilibration against precipitant solution, X-ray diffraction strcuture determination and analysis at 3.1 A resolution, modeling Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ essential for activity, coordinated to a His-side chain of the active site Escherichia coli
Zn2+ essential for activity, coordinated to a His-side chain of the active site, mechanistic role Homo sapiens

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Homo sapiens P30793
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant DELTA42-hGTP-CH-I from Escherichia coli M15 Homo sapiens

Reaction

Reaction Comment Organism Reaction ID
GTP + H2O = formate + 7,8-dihydroneopterin 3'-triphosphate enzyme contains to thiol groups per active site, reaction mechanism Escherichia coli
a
GTP + H2O = formate + 7,8-dihydroneopterin 3'-triphosphate The reaction involves hydrolysis of two C-N bonds and isomerization of the pentose unit, the recyclization may be non-enzymic, reaction mechanism Homo sapiens
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O
-
 Escherichia coli formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate
-
 ?
GTP + H2O
-
 Homo sapiens formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate
-
 ?

Subunits

Subunits Comment Organism
decamer each subunit contains 1 active site Escherichia coli

Synonyms

Synonyms Comment Organism
GTP-CH-I
-
 Escherichia coli
GTP-CH-I
-
 Homo sapiens