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Literature summary for 3.5.4.16 extracted from

  • Schramek, N.; Bracher, A.; Fischer, M.; Auerbach, G.; Nar, H.; Huber, R.; Bacher, A.
    Reaction mechanism of GTP cyclohydrolase I: single turnover experiments using a kinetically competent reaction intermediate (2002), J. Mol. Biol., 316, 829-837.
    View publication on PubMed
Search for more literature for 3.5.4.16

Protein Variants

Protein Variants Comment Organism
H179N mutant H179N is not able to perform the whole reaction step, but can catalyzes the reversible formation of reaction intermediate 2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics of the reaction steps, single turnover experiments, wild-type and H179N mutant enzymes, overview Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GTP + H2O Escherichia coli first commited step in the biosynthesis of tetrahydrofolate and tetrahydrobiopterin formate + D-erythro-dihydroneopterin triphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Reaction

Reaction Comment Organism Reaction ID
GTP + H2O = formate + 7,8-dihydroneopterin 3'-triphosphate reaction mechanism involving reaction intermediates Escherichia coli
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + H2O first commited step in the biosynthesis of tetrahydrofolate and tetrahydrobiopterin Escherichia coli formate + D-erythro-dihydroneopterin triphosphate
-
 ?
GTP + H2O enzyme catalyzed opening of the imidazole ring of GTP, kinetically competent reaction intermediate is 2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone Escherichia coli formate + D-erythro-dihydroneopterin triphosphate
-
 ?
GTP + H2O mutant H179N is not able to perform the whole reaction step Escherichia coli 2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone triphosphate + ?
-
 r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 assay at Escherichia coli