Protein Variants | Comment | Organism |
---|---|---|
E64A | E64A mutation causes a decrease in kcat of 5 orders of magnitude and an increase in Km of 2 orders of magnitude, resulting in a decrease in kcat/Km of 8 orders of magnitude | Saccharomyces cerevisiae |
E64D | the effect of E64D mutation are slightly milder than the E64A mutation | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-fluoro-1H-pyrimidin-2-one | 19F NMR experiments show that binding of the inhibitor 5-fluoro-1H-pyrimidin-2-one (5FPy) to the wild-type yCD causes an upfield shift, indicating that the bound inhibitor is in the hydrated form, mimicking the transition state or the tetrahedral intermediate in the activation of 5FC. Binding of 5FPy to the E64A mutant enzyme causes a downfield shift, indicating that the bound 5FPy remains in an unhydrated form in the complex with the mutant enzyme | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.16 | - |
5-fluorocytosine | wild-type, pH 7.5, temperature not specified in the publication | Saccharomyces cerevisiae | |
8 | 9 | 5-fluorocytosine | mutant E64A, pH 7.5, temperature not specified in the publication | Saccharomyces cerevisiae | |
19 | - |
5-fluorocytosine | mutant E64D, pH 7.5, temperature not specified in the publication | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-fluorocytosine + H2O | - |
Saccharomyces cerevisiae | 5-fluorouracil + NH3 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
yCD | - |
Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00013 | - |
5-fluorocytosine | mutant E64A, pH 7.5, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.005 | - |
5-fluorocytosine | mutant E64D, pH 7.5, temperature not specified in the publication | Saccharomyces cerevisiae | |
17 | - |
5-fluorocytosine | wild-type, pH 7.5, temperature not specified in the publication | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Saccharomyces cerevisiae |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00086 | - |
5-fluoro-1H-pyrimidin-2-one | wild-type, pH 7.5, temperature not specified in the publication | Saccharomyces cerevisiae | |
1.06 | - |
5-fluoro-1H-pyrimidin-2-one | mutant E64D, pH 7.5, temperature not specified in the publication | Saccharomyces cerevisiae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0000014 | - |
5-fluorocytosine | mutant E64A, pH 7.5, temperature not specified in the publication | Saccharomyces cerevisiae | |
0.00026 | - |
5-fluorocytosine | mutant E64D, pH 7.5, temperature not specified in the publication | Saccharomyces cerevisiae | |
106 | - |
5-fluorocytosine | wild-type, pH 7.5, temperature not specified in the publication | Saccharomyces cerevisiae |